Monoclonal Antibody against Yellowtail Thymic Lymphocytes Recognizing a Lymphocytic Subpopuation

DOI Web Site 参考文献3件
  • Nishimura Hitoshi
    Fish Disease Laboratory, Faculty of Agricultre, Kochi University Laboratory of Host Defense and Germfree Life, Nagoya University School of Medicine
  • Akamatsu Norihiko
    Fish Disease Laboratory, Faculty of Agricultre, Kochi University
  • Ikemoto Masaru
    Laboratory of Aquatic Bioligy, Faculty of Agriculture, Kyoto University
  • Kawai Kenji
    Fish Disease Laboratory, Faculty of Agricultre, Kochi University
  • Miyazawa Hitoshi
    Department of Immunology, Kochi Medical School
  • Fujimoto Shigeyoshi
    Department of Immunology, Kochi Medical School
  • Kusuda Riichi
    Fish Disease Laboratory, Faculty of Agricultre, Kochi University

抄録

Monoclonal antibody (mAb) YeT-2 was generated in mice hyper-immunized with thym c lympho-cytes of yellowtail Seriola quinqueradiata. The mAb YeT-2 reacted withthe major population of peripheral blood lymphocytes, but not with serum IgM. OnPercoll density gradientcentrifugation, YeT-2 positive cells were distributed at densities of 1.058 and 1.064. The latter density layer also included some YeT-2 negative cells. Furthermore, on electron microscopic studies, YeT-2 positivelymphocytes in peripheral blood could not be distinguished from YeT-2 positivelymphocytes in thymus. Flow cytometric analysis showed that mAb YeT-2 positivelymphocytes comprised 88.4% and 76.1% of the peripheral blood and thymus, respectively. Functional studies revealed that YeT-2 positive lymphocytes respond to concanavalin A but not to lipopolysaccharide, while YeT-2 negative lymphocytes responded in the opposite way. From these results, it can be concluded that the novel mAb YeT-2 recognizes a lymphocytic population which might be the putative T cell.

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