OK‐432による癌性胸・腹水中へのTNFの誘導とそのヒト肺癌培養細胞に対する抗腫よう効果について  [in Japanese] Induction of TNF activity in the carcinomatous pleural effusion and ascites by direct injection of OK-432 and it's anti-tumor effects on human lung cancer cell lines.  [in Japanese]

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癌性胸・腹水中にOK-432を投与することにより, L細胞や各種肺癌培養細胞に対し強い細胞障害性を有する物質が得られた.また, ヌードマウスへの移植腫瘍に対しても発育阻止および延命を示したが, 正常細胞には障害を示さなかった.この物質は分子量14000~18000で, 56℃で30分の熱処理および部分精製にても失活せず, ヒトTNFであることが示唆された.ヒトTNFおよびTNF誘導物質の投与は肺癌治療の有効な方法と考えられた.

Potent cytotoxic activity against L cells as well as a number of cultured human lung cancer cell lines was found in the carcinomatous pleural effusion and ascites afterintrapleural or intraperitoneal injections of OK-432. Such effusions also showed to inhibit the transplanted tumor growth in nude mice and induce significant prolongation of survival time of these mice, whereas they showed no effect on normal cells at all.<BR>The activity was detected at around 14-18 Kd MW by partial purification of DEAE-A50 and Sephadex G-200 and was resistant to heat treatment at 56°Cfor 30 min., suggesting strongly that the activity was mediated by TNF.<BR>It was considered that TNF as well as TNF-inducing agents such as OK-432 could be an effective agent for managing human lung cancers.


  • Haigan

    Haigan 28(3), 289-295, 1988

    The Japan Lung Cancer Society


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