炭酸ガスレーザー照射によるラットけい骨の骨形成促進に関する研究

• 塩崎 洋堂

  明海大学歯学部歯科放射線学講座

• 横瀬 敏志

  奥羽大学歯学部保存修復学講座

• 齋藤 正樹

  明海大学歯学部保存修復学講座

• 鈴木 洋

  明海大学歯学部保存修復学講座

• 廣瀬 直子

  明海大学歯学部保存修復学講座

• 清水 英之

  明海大学歯学部歯科放射線学講座

• 益田 賢治

  明海大学歯学部歯科放射線学講座

• 長谷川 彰彦

  明海大学歯学部総合臨床医学第一講座(内科学)

• 片山 直

  明海大学歯学部保存修復学講座

• 奥村 泰彦

  明海大学歯学部歯科放射線学講座

書誌事項

タイトル別名

• Carbon Dioxide Laser Irradiation Stimulates Bone Formation in Rat Tibiae

抄録

Low Level Laser Therapy (LLLT) using CO₂ lasers is known to activate surrounding cells and tissue. However, few studies concerning LLLT using hard lasers have been perform, and much about the biological mechanism of LLLT. In the present study, we report morphological changes in rat tibiae irradiated by CO₂ laser without surgical treatment. The purpose of this study was to histologically evaluate the effects of LLLT using a CO₂ laser on bone formation in rat tibiae. The left tibiae underwent CO₂ laser irradiation, while the right tibia did not undergo CO₂ laser irradiatio (Control). Rat tibiae were irradiated by CO₂ laser from the outside of the body.Irradiation was performed at the following power and exposure time: power, LOW; duration: 15 min; calculated energy density, -1.1KJ/cm₂. On the 7th day after irradiation, tibiae were removed and were analyzed by Soft X-ray. Serial sections were prepared for osteoid staining (Yoshiki's method) and ALP (Alkalinephosphatase) staining, and were examined for mineralized apposition rate (Calcein labeling). Soft X-ray examination revealed no differences between the control and CO₂ laser-irradiated groups. However, marked osteoid formation (Yoshiki's method) on the surface of laser-irradiated tibiae and young ALP-positive osteoblasts were present in the osteoid tissue. Interestingly, calcein labeling demonstrated that mineral apposition rate on the periosteum of laser-irradiated cortical bone was much higher than that in control tibia. These results demonstrate that LLLT using CO₂ laser induced not only mineralization but also osteoblast differentiation. The present study suggests that CO₂ laser can be applied to bone regeneration treatment. (J. Jpn. Soc. Laser Dent. 16: 23-30, 2005 Reprint request to Dr. YOKOSE) Key words=LLLT, Bone regeneration, CO₂ laser

収録刊行物

• 日本レーザー歯学会誌

  日本レーザー歯学会誌 16 (1), 23-30, 2005

  日本レーザー歯学会

キーワード

• LLLT
• 骨再生
• 炭酸ガスレーザー

詳細情報

• CRID: 1390282679450803072
• NII論文ID: 130003925115
• DOI: 10.5984/jjpnsoclaserdent.16.23
• ISSN: 21856702
• ISSN: 09177450
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可