CELL-MEDIATED AND HUMORAL IMMUNITY IN MICE: CROSS REACTION BETWEEN LYSOZYME AND S-CARBOXYMETHYLATED LYSOZYME STUDIED BY A MODIFIED FOOTPAD TEST

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Abstract

The mouse sensitized by subcutaneous (sc) injection of lysozyme in emulsion of Freund's complete adjuvant (FCA) was shown by a modified footpad test to develop three kinds of hypersensitivities. Injecting lysozyme in 2.5-μ1 emulsion of Freund's incomplete adjuvant (FIA) into the footpad elicited strong footpad swelling in 30 min (anaphylactic reaction), in 3 hr (Arthus-type reaction) and in 24 hr (delayed-type hypersensitivity; DTH) . The mice showing anaphylactic reaction in the footpad test manifested severe active systemic anaphylaxis, and the sera of these animals showed high IgG<SUB>1</SUB> antibody titers with only sparingly detectable or no IgE antibody titers.<BR>In the sensitizing system with the use of FCA, the antigenicity of S-carboxy-methylated lysozyme (CM-lysozyme) devoid of the three-dimensional conformation of lysozyme was compared with that of the native molecule. CM-lysozyme and lysozyme completely cross-reacted to each other in DTH, but not at all in the anaphylactic or Arthus-type reaction or in IgG<SUB>1</SUB> antibody production. CM-lysozyme was shown also to have the ability to bestow immunological memory for the induction of humoral immunity against lysozyme; intravenous (iv) injection of lysozyme in saline or sc injection of CM-lysozyme-FCA alone failed to induce immediate hypersensitivities and IgG<SUB>1</SUB> antibody production against lysozyme, but pre-sensitization by sc injection of CM-lysozyme-FCA enabled the animal to induce these responses to significant levels when iv injection of lysozyme in saline was given as a booster.

Journal

  • Japanese Journal of Medical Science and Biology

    Japanese Journal of Medical Science and Biology 28(1), 23-35, 1975

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

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