Mass Identification of Chloroplast Proteins of Endosymbiont Origin by Phylogenetic Profiling Based on Organism-Optimized Homologous Protein Groups
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- Sato Naoki
- Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo
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- Ishikawa Masayuki
- Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo
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- Fujiwara Makoto
- Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo
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- Sonoike Kintake
- Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo
Abstract
Chloroplasts originate from ancient cyanobacteria-like endosymbiont. Several tens of chloroplast proteins are encoded by the chloroplast genome, while more than hundreds are encoded by the nuclear genome in plants and algae, but the exact number and identity of nuclear-encoded chloroplast proteins are still unknown. We describe here attempts to identify a large number of unidentified chloroplast proteins of endosymbiont origin (CPRENDOs). Our strategy consists of whole genome protein clustering by the homolog group method, which is optimized for organismnumber, and phylogenetic profiling that extract groups conserved in cyanobacteria and photosynthetic eukaryotes. An initial minimal set of CPRENDOs was predicted without targeting prediction and experimentally validated.
Journal
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- Genome Informatics
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Genome Informatics 16 (2), 56-68, 2005
Japanese Society for Bioinformatics
