Immunobiological activities of sodium dodecylsulfatesoluble fractions derived from the cell envelopes of oral gram-negative, anaerobic rods

• Kato Keijiro

  Department of Oral Microbiology, Okayama University

• Kokeguchi Susumu

  Department of Oral Microbiology, Okayama University

• Ishihara Hiroko

  Department of Oral Microbiology, Okayama University

• Fukui Kazuhiro

  Department of Oral Microbiology, Okayama University

• Tsujimoto Masachika

  Department of Microbiology and Oral Microbiology, Osaka University

• Takada Haruhiko

  Department of Microbiology and Oral Microbiology, Osaka University

• Ogawa Tomohiko

  Department of Microbiology and Oral Microbiology, Osaka University

• Kotani Shozo

  Department of Microbiology and Oral Microbiology, Osaka University

• Kinoshita Masahiko

  Department of Periodontology and Endodontology, Okayama University

• Kurihara Hidemi

  Department of Periodontology and Endodontology, Okayama University

• Nomura Yoshio

  Department of Periodontology and Endodontology, Okayama University

• Murayama Yoji

  Department of Periodontology and Endodontology, Okayama University

• Umemoto Toshio

  Department of Oral Microbiology, Kanagawa Dental College

Bibliographic Information

Other Title

• 口こう内グラム陰性けん気性かん菌のセルエンベロープからドデシル硫酸ナトリウムで可溶化される画分の免疫，生物学的活性

Abstract

1. Chemical composition and immunobiological activities of sodium dodecylsulfate (SDS)-soluble fractions of cell envelopes derived from gram-negative, anaerobic rods (A. actinomyceterncomitans ATCC 29522, ATCC 29524, Y4, B. gingivalis #381, #1021, B. asaccharolyticus ATCC 27067 and F. nucleatum ATCC 25586) were investigated.<BR>2. Main components of the preparations were protein (42-78%) and lipid (as fatty acid, 16-27%, except F. nucleatum, 10%). The preparations were free from peptidoglycan in terms of muramic acid determination and the content of 2-keto-3-deoxy-octonate (KDO) and endotoxin (in terms of toxicolor®test) was less than 0.11 and 0.02%, respectively.<BR>3. The preparations which exhibited induction of delayed-type hypersensitivity and antibody production were A. actinomycetemcomitans ATCC 29524 and B. gingivalis #381, #1021, and the preparations of A. actinomycetemcomitans ATCC 29522 and B. asaccharolyticus ATCC 27067 exhibited only potentiation of cell-mediated immunity.<BR>4. Mitogenic activities of the preparations were investigated by using culturing splenocytes of athymic BALB/c nu/nu, C3H/HeN and C3H/HeJ mice, and all of the preparations tested were proved to be potent B cell mitogen.<BR>5. Migration-stimulating activities of the preparations on human peripheral blood monocytes were investigated, and all of the preparations other than A. actinomycetemcomitans ATCC 29524 and B. gingivalis #1021 exhibited lower but definite activities than those of positive control, FLMP and LPS-activated serum.<BR>6. Effects of the SDS-soluble fractions on the morphology and [³H]-thymidine incorporation of human fibroblasts were investigated. Morphological changes were observed in concentration of 100μg/ml in order of B. gingivalis #381, #1021>F. nucleatum>B. asaccharolyticus>A. actinomycetemcomitans ATCC 29524. The preparations of A. actinomycetemcomitans in concentrations less than 20 μg/ml exhibited slightly potent inhibitory effect on incorporation of [³H]-thymidine than those of B. gingivalis and B. asaccharolyticus.

Journal

• Japanese Journal of Oral Biology

  Japanese Journal of Oral Biology 26 (3), 640-655, 1984

  Japanese Association for Oral Biology

Keywords

• gram-negative anaerobe
• cell envelope
• SDS-soluble component
• immunomodulation
• fibroblast

Details

• CRID: 1390282679659787776
• NII Article ID: 130004001264
• DOI: 10.2330/joralbiosci1965.26.640
• ISSN: 03850137
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed