超臨界流体と高分子　ＩＩ　　超臨界流体クロマトグラフィーによるポリプレノールの構造解析

• 馬場 健史

  大阪大学大学院工学研究科

• 福崎 英一郎

  大阪大学大学院工学研究科

• 中沢 慶久

  日立造船 (株)

• 佐藤 浩昭

  名城大学農学ハイテクリサーチセンター

• 右手 浩一

  大阪大学大学院基礎工学研究科

• 北山 辰樹

  大阪大学大学院基礎工学研究科

• 小林 昭雄

  大阪大学大学院工学研究科

書誌事項

タイトル別名

• Supercritical Fluid in Polymer Science and Technology. II. Analysis of Polyprenols by Supercritical Fluid Chromatography.

抄録

Applications of supercritical fluid chromatography (SFC) to the analysis of plant polyprenols are described. By the use of SFC, chromatographic resolution of polyprenol homologs and their geometric isomers was improved markedly as compared with that obtained by the use of conventional high-performance liquid chromatography (HPLC). Under optimized SFC conditions, individual homologs from 10 mer to 100 mer were separated. It was also possible to isolate each geometric isomer of polypernol homologs from 13 mer to 20 mer by the fractionation using SFC. The chain-length distributions of polyprenol samples determined by SFC agreed essentially with those determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). SFC analysis of polyprenols extracted from the leaf, root and seed coat of a rubber-producing plant, Eucommia ulmoides Oliver (E. ulmoides), has revealed the presence of all-trans polyprenols with degrees of polymerizations larger than 10, for the first time in nature. The all-trans polyprenols had broad distributions of chain-lengths leading to high molecular weight, which suggests that the polyprenols should act as biosynthesis intermediates for the high molecular weight trans-1, 4-polyisoprene in this plant. The chain-length distributions of the trans polyprenols from the leaf, root and seed coat differed from each other, suggesting the presence of site-specific control mechanisms of chain-termination. On the other hand, cispolyprenols have been found to occur in all parts of E. ulmoides. They had narrow chain-length distributions similar to those of dolichols.

収録刊行物

• 高分子論文集

  高分子論文集 58 (12), 642-649, 2001

  公益社団法人 高分子学会

キーワード

• Supercritical Fluid Chromatography
• High-Performance Liquid Chromatography
• Polyprenol
• Geometric Isomer
• Chain-Length Distribution
• <I>Eucommia ulmoides</I> Oliver
• Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
• Nuclear Magnetic Resonance

詳細情報

• CRID: 1390282681499596160
• NII論文ID: 130004035469
• DOI: 10.1295/koron.58.642
• ISSN: 18815685
• ISSN: 03862186
• ISSN: http://id.crossref.org/issn/03862186
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可