角柱状切片の利用による三次元電子顕微鏡像の改善

• 金子 武司

  京都工芸繊維大学大学院工芸科学研究科高分子学専攻

• 西川 幸宏

  京都工芸繊維大学大学院工芸科学研究科高分子学専攻

• 西岡 秀夫

  (財) 化学技術戦略推進機構・京都工芸繊維大学大学院工芸科学研究科

• 西 敏夫

  東京工業大学大学院理工学研究科有機・高分子物質専攻

• 陣内 浩司

  京都工芸繊維大学大学院工芸科学研究科高分子学専攻

書誌事項

タイトル別名

• Improvement of 3D Images by Use of Prism-shaped Sections

抄録

A quadrangular prism specimen made of a block copolymer nanostructure (a ‘prism-shaped’ section) was observed in three dimensions (3D) by transmission electron microtomography (TEMT) over as wide a tilt range as possible. A cross section of the prism-shaped section was 200 nm on each side. For comparison, a conventional ultra-thin section having the same thickness as the prism-shaped section was employed. The image quality of the projections taken at a high tilt angle, e. g. 60°, of the prism-shaped section was better than that of the ultra-thin section because its effective thickness was thinner than the ultra-thin section at the same tilt angle. As a result, the projections at very high tilt angles, e. g. ±75°, can be used for the 3D reconstruction in the case of prism-shaped sections, while those at such high tilt angles obtained from the ultra-thin section become so blurred that they may not be appropriate for use in the reconstruction. By use of a prism-shaped section, the contrast of the reconstructed image was significantly enhanced. The elongation of the images along the optical axis of microscope due to the Missing Wedge was also considerably reduced in the case of the prism-shaped section.

収録刊行物

• 高分子論文集

  高分子論文集 62 (10), 508-513, 2005

  公益社団法人 高分子学会

キーワード

• Electron Tomography
• Three-dimensional Reconstruction
• Block Copolymer
• Gyroid Structure
• Droplet

詳細情報

• CRID: 1390282681499230464
• NII論文ID: 130004035694
• DOI: 10.1295/koron.62.508
• ISSN: 18815685
• ISSN: 03862186
• ISSN: http://id.crossref.org/issn/03862186
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可