Mitosis-Coupled, Microtubule-Dependent Clustering of Endosomal Vesicles around Centrosomes

• Takatsu Hiroyuki

  Graduate School of Pharmaceutical Sciences, Kyoto University Career-Path Promotion Unit for Young Life Scientists, Kyoto University

• Katoh Yohei

  Graduate School of Pharmaceutical Sciences, Kyoto University

• Ueda Tomoko

  Graduate School of Pharmaceutical Sciences, Kyoto University

• Waguri Satoshi

  Department of Anatomy and Histology, Fukushima Medical University School of Medicine

• Murayama Takashi

  Department of Pharmacology, Juntendo University School of Medicine

• Takahashi Senye

  Graduate School of Pharmaceutical Sciences, Kyoto University

• Shin Hye-Won

  Career-Path Promotion Unit for Young Life Scientists, Kyoto University Graduate School of Pharmaceutical Sciences, Kyoto University

• Nakayama Kazuhisa

  Graduate School of Pharmaceutical Sciences, Kyoto University

Abstract

Upon cell division, not only cells themselves but also their organelles undergo drastic shape changes, although the behaviors of organelles other than the Golgi apparatus remain poorly understood. We followed the spatiotemporal changes in the localization of transferrin receptor (TfnR) and other proteins. In early mitotic phases, a population of proteins cycling through the endocytic recycling compartment (ERC) exhibits a distinct spatiotemporal change from that of Golgi proteins. In prophase/prometaphase, when the cell surface-to-volume ratio is reaching its minimum, the ERC proteins are transiently assembled around the centrated centrosome in a microtubule- and dynein-dependent manner, and soon separated polewards into two clusters concomitant with separation of duplicated centrosomes. Electron microscopic analysis revealed that endosomal vesicles containing endocytosed transferrin cluster tightly around centrosomes without fusing with one another. As cytokinesis proceeds, the clusters gradually collapse, and the ERC proteins reassemble around the furrowing equatorial region. FRAP (fluorescence recovery after photobleaching) analyses of EGFP-TfnR-expressing cells revealed minimal membrane exchange between the endosomal clusters and other cellular compartments until anaphase/telophase, when membrane traffic resumes. Our observations indicate that ERC clustering around centrosomes plays a fundamental role in restricting membrane delivery to the plasma membrane during early mitotic phases, when the cell surface-to-volume ratio reaches its minimum.

Journal

• Cell Structure and Function

  Cell Structure and Function 38 (1), 31-41, 2013

  Japan Society for Cell Biology

Keywords

• recycling endosome
• transferrin receptor
• spindle pole
• cytokinesis
• dynein

Details

• CRID: 1390001204695042816
• NII Article ID: 130004053889
• NII Book ID: AA0060007X
• DOI: 10.1247/csf.12028
• COI: 1:STN:280:DC%2BC3szgtl2hsQ%3D%3D
• ISSN: 13473700
• ISSN: 03867196
• NDL BIB ID: 025316074
• PubMed: 23328347
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025316074
• Web Site: https://www.jstage.jst.go.jp/article/csf/38/1/38_12028/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015047627
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed