Cell Density-dependent Nuclear Accumulation of ELK3 is Involved in Suppression of PAI-1 Expression

• Tanaka Shu

  Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University

• Nakao Kazuyuki

  Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University

• Sekimoto Toshihiro

  Department of Biochemistry, Graduate School of Medicine, Osaka University

• Oka Masahiro

  Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University Department of Biochemistry, Graduate School of Medicine, Osaka University

• Yoneda Yoshihiro

  Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University Department of Biochemistry, Graduate School of Medicine, Osaka University

Abstract

Cell-cell contact regulates the proliferation and differentiation of non-transformed cells, e.g., NIH/3T3 cells show growth arrest at high cell density. However, only a few reports described the dynamic behavior of transcription factors involved in this process. In this study, we showed that the mRNA levels of plasminogen activator inhibitor type 1 (PAI-1) decreased drastically at high cell density, and that ELK3, a member of the Ets transcription factor family, repressed PAI-1 expression. We also demonstrated that while ELK3 was distributed evenly throughout the cell at low cell density, it accumulated in the nucleus at high cell density, and that binding of DNA by ELK3 at the A domain facilitated its nuclear accumulation. Furthermore, we found that ETS1, a PAI-1 activator, occupied the ELK3-binding site within the PAI-1 promoter at low cell density, while it was released at high cell density. These results suggest that at high cell density, the switching of binding of transcription factors from ETS1 to ELK3 occurs at a specific binding site of the PAI-1 promoter, leading to the cell-density dependent suppression of PAI-1 expression.

Journal

• Cell Structure and Function

  Cell Structure and Function 38 (2), 145-154, 2013

  Japan Society for Cell Biology

Keywords

• High cell density
• transcription factors
• nucleocytoplasmic transport

Details

• CRID: 1390001204696888192
• NII Article ID: 130004053896
• NII Book ID: AA0060007X
• DOI: 10.1247/csf.13007
• COI: 1:STN:280:DC%2BC3snmvV2gtw%3D%3D
• ISSN: 13473700
• ISSN: 03867196
• NDL BIB ID: 025316295
• PubMed: 23708702
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025316295
• Web Site: https://www.jstage.jst.go.jp/article/csf/38/2/38_13007/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015047635
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed