Chromosomes Rein Back the Spindle Pole Body during Horsetail Movement in Fission Yeast Meiosis

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Author(s)

    • Yamane Miho Yamane Miho
    • Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology
    • Mori Chie
    • Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology
    • Fukuta Noriko
    • Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology
    • Matsuda Atsushi
    • Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology|Graduate School of Frontier Biosciences, Osaka University
    • Haraguchi Tokuko
    • Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology|Graduate School of Frontier Biosciences, Osaka University
    • Hiraoka Yasushi
    • Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology|Graduate School of Frontier Biosciences, Osaka University

Abstract

In meiosis, pairing and recombination of homologous chromosomes are crucial for the correct segregation of chromosomes, and substantial movements of chromosomes are required to achieve homolog pairing. During this process, it is known that telomeres cluster to form a bouquet arrangement of chromosomes. The fission yeast <i>Schizosaccharomyces pombe</i> provides a striking example of bouquet formation, after which the entire nucleus oscillates between the cell poles (these oscillations are generally called horsetail nuclear movements) while the telomeres remain clustered to the spindle pole body (SPB; a centrosome-equivalent structure in fungi) at the leading edge of the moving nucleus. <i>S. pombe</i> mutants defective in telomere clustering frequently form aberrant spindles, such as monopolar or nonpolar spindles, leading to missegregation of the chromosomes at the subsequent meiotic divisions. Here we demonstrate that such defects in meiotic spindle formation caused by loss of meiotic telomere clustering are rescued when nuclear movement is prevented. On the other hand, stopping nuclear movement does not rescue defects in telomere clustering, nor chromosome missgregation even in cells that have formed a bipolar spindle. These results suggest that movement of the SPB without attachment of telomeres leads to the formation of aberrant spindles, but that recovering bipolar spindles is not sufficient for rescue of chromosome missegregation in mutants lacking telomere clustering.

Journal

  • Cell Structure and Function

    Cell Structure and Function 39(2), 93-100, 2014

    Japan Society for Cell Biology

Codes

  • NII Article ID (NAID)
    130004053902
  • NII NACSIS-CAT ID (NCID)
    AA0060007X
  • Text Lang
    ENG
  • ISSN
    0386-7196
  • NDL Article ID
    026678591
  • NDL Call No.
    Z53-V38
  • Data Source
    NDL  J-STAGE 
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