Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae

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Author(s)

    • Yamagishi Kazuo
    • Department of Biotechnology, Graduate School of Engineering, Osaka University
    • Sugiyama Minetaka
    • Department of Biotechnology, Graduate School of Engineering, Osaka University
    • Kaneko Yoshinobu
    • Department of Biotechnology, Graduate School of Engineering, Osaka University
    • Harashima Satoshi
    • Department of Biotechnology, Graduate School of Engineering, Osaka University

Abstract

Based on a previously developed PCR-mediated chromosome splitting method, a genome engineering technique was developed in haploid <i>Saccharomyces cerevisiae</i> for incorporating any desired chromosomal region into a chromosome that carries a single gene. Based on the viability of cells carrying an essential gene in such a construct, close physical proximity of two telomeres and a centromere does not appear to compromise gene function. Spontaneous loss of constructed single-gene chromosomes during vegetative growth was high (0.2–0.4 per cell division), suggesting the possibility of creating novel cells carrying single-gene chromosomes derived from various chromosomal regions in a variety of combinations by exploiting combinatorial loss.<br>

Journal

  • Journal of Bioscience and Bioengineering

    Journal of Bioscience and Bioengineering 106(6), 563-567, 2008

    The Society for Biotechnology, Japan

Codes

  • NII Article ID (NAID)
    130004056658
  • Text Lang
    ENG
  • ISSN
    1389-1723
  • Data Source
    J-STAGE 
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