Enhanced Release of Chitosanase from Streptomyces griseus through Direct Interaction of Liposome with Cell Membrane under Heat Stress

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Author(s)

    • Ngo Kien Xuan
    • Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
    • Umakoshi Hiroshi
    • Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
    • Shimanouchi Toshinori
    • Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
    • Bui Huong Thi
    • Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
    • Kuboi Ryoichi
    • Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University

Abstract

A direct interaction of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes with membrane of <i>Streptomyces griseus</i> cell under the heat stress at 41°C increased the chitosanase production and release to 2.2 times higher than that at 37°C without the POPC liposomes. Amount of chitosanase released across the lipid mimicking cell membrane (LMCM) liposome under a heat at 41°C in the presence of POPC liposomes was 17% of initially-entrapped chitosanase while it was only 1% in the absence of POPC liposomes, even under a heat stress at 41°C, clearly showing the importance of the direct interaction between membrane and membrane.<br>

Journal

  • Journal of Bioscience and Bioengineering

    Journal of Bioscience and Bioengineering 106(6), 602-605, 2008

    The Society for Biotechnology, Japan

Codes

  • NII Article ID (NAID)
    130004056665
  • Text Lang
    ENG
  • ISSN
    1389-1723
  • Data Source
    J-STAGE 
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