Potential of a mutant-based reverse genetic approach for functional genomics and molecular breeding in soybean

• Anai Toyoaki

  Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Saga University

Abstract

Mutant-based reverse genetics offers a powerful way to create novel mutant alleles at a selected locus. This approach makes it possible to directly identify plants that carry a specific modified gene from the nucleotide sequence data. Soybean [Glycine max (L.) Merr.] has a highly redundant paleopolyploid genome (approx. 1.1 Gb), which was completely sequenced in 2010. Using reverse genetics to support functional genomics studies designed to predict gene function would accelerate post-genomics research in soybean. Furthermore, the novel mutant alleles created by this approach would be useful genetic resources for improving various traits in soybean. A reverse genetic screening platform in soybean has been developed that combines more than 40,000 mutant lines with a high-throughput method, Targeting Local Lesions IN Genome (TILLING). In this review, the mutant-based reverse genetic approach based on this platform is described, and the likely evolution of this approach in the near future.<br>

Journal

• Breeding Science

  Breeding Science 61 (5), 462-467, 2012

  Japanese Society of Breeding

Keywords

• reverse genetics
• mutagenesis
• TILLING
• soybean

Details

• CRID: 1390282679699250816
• NII Article ID: 130004057127
• NII Article ID: 10030432780
• NII Book ID: AA11317194
• DOI: 10.1270/jsbbs.61.462
• COI: 1:CAS:528:DC%2BC38Xpt1yhtrY%3D
• ISSN: 13473735
• ISSN: 13447610
• NDL BIB ID: 023526835
• PubMed: 23136486
• Web Site: http://id.ndl.go.jp/bib/023526835
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023526835
• Web Site: https://www.jstage.jst.go.jp/article/jsbbs/61/5/61_5_462/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed