Suppressive Potential of <i>Paenibacillus</i> Strains Isolated from the Tomato Phyllosphere against Fusarium Crown and Root Rot of Tomato

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  • Sato Ikuo
    Environmental Biofunction Division, National Institute for Agro-Environmental Sciences
  • Yoshida Shigenobu
    Environmental Biofunction Division, National Institute for Agro-Environmental Sciences
  • Iwamoto Yutaka
    Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries
  • Aino Masataka
    Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries
  • Hyakumachi Mitsuro
    Faculty of Applied Biosciences, Gifu University
  • Shimizu Masafumi
    Faculty of Applied Biosciences, Gifu University
  • Takahashi Hideki
    Graduate School of Agricultural Science, Tohoku University
  • Ando Sugihiro
    Graduate School of Agricultural Science, Tohoku University
  • Tsushima Seiya
    Natural Resources Inventory Center, National Institute for Agro-Environmental Sciences

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Other Title
  • Suppressive Potential of Paenibacillus Strains Isolated from the Tomato Phyllosphere against Fusarium Crown and Root Rot of Tomato

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Abstract

The suppressive potentials of Bacillus and Paenibacillus strains isolated from the tomato phyllosphere were investigated to obtain new biocontrol candidates against Fusarium crown and root rot of tomato. The suppressive activities of 20 bacterial strains belonging to these genera were examined using seedlings and potted tomato plants, and two Paenibacillus strains (12HD2 and 42NP7) were selected as biocontrol candidates against the disease. These two strains suppressed the disease in the field experiment. Scanning electron microscopy revealed that the treated bacterial cells colonized the root surface, and when the roots of the seedlings were treated with strain 42NP7 cells, the cell population was maintained on the roots for at least for 4 weeks. Although the bacterial strains had no direct antifungal activity against the causal pathogen in vitro, an increase was observed in the antifungal activities of acetone extracts from tomato roots treated with the cells of both bacterial strains. Furthermore, RT-PCR analysis verified that the expression of defense-related genes was induced in both the roots and leaves of seedlings treated with the bacterial cells. Thus, the root-colonized cells of the two Paenibacillus strains were considered to induce resistance in tomato plants, which resulted in the suppression of the disease.

Journal

  • Microbes and Environments

    Microbes and Environments 29 (2), 168-177, 2014

    Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

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