Comparison of Gelsolin Purification Performance between Anion-exchange-graft-chain-containing Porous Membrane and Anion-exchange Bead-packed Bed

DOI
  • YOSHIKAWA Takashi
    Department of Applied Chemistry and Biotechnology, Chiba University
  • HAGIWARA Kyohei
    Department of Applied Chemistry and Biotechnology, Chiba University
  • SAITO Kyoichi
    Department of Applied Chemistry and Biotechnology, Chiba University
  • KATAYAMA Eisaku
    Division of Biomolecular Imaging, Institute of Medical Science, The University of Tokyo
  • TOJO Tadashi
    Division of Biomolecular Imaging, Institute of Medical Science, The University of Tokyo
  • SUGO Takanobu
    Environment Purification Research Institute

Bibliographic Information

Other Title
  • ｇｅｌｓｏｌｉｎ精製におけるアニオン交換グラフト鎖搭載多孔性膜とアニオン交換ビーズカラムとの性能比較

Abstract

Proteins containing gelsolin in bovine plasma were bound to anion-exchange porous hollow-fiber membrane before gelsolin was specifically eluted with a calcium ion-containing solution. The epoxy group introduced into the polymer chain grafted onto the porous hollow-fiber membrane was converted into two kinds of anion-exchange groups, i.e., diethylamino (DEA) and 2-hydroxyethylamino (HEA) groups with various mole fractions. The porous membrane containing exclusively HEA group exhibited the highest liquid permeability and the largest amount of gelsolin recovered. Furthermore, the HEA-group-containing porous membrane was advantageous over a column charged with commercially available anion-exchange beads in that the amount of gelsolin recovered was constant irrespective of space velocity, i.e., residence time across the membrane thickness, of the protein solution.

Journal

Details 詳細情報について

  • CRID
    1390001204434264320
  • NII Article ID
    130004061613
  • DOI
    10.5182/jaie.18.2
  • ISSN
    18843360
    0915860X
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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