プラセンターリキッドのチロシナーゼ阻害力及びアルカリフォスファターゼ活性の保持性

DOI

書誌事項

タイトル別名
  • Preservations of Tyrosinase Inhibitory and Alkaline Phosphatase Activities in Placentaliquid

抄録

Placentaliquid has been graded by alkaline phosphatase activity. Other various components are contained in placenta and several biological activties have been reported. However, the relationship between the biological activities and the components has not been made clear yet.<br>Storaged Placentaliquid was tested for the preservation ability of the alkaline phosphatase activity. The alkaline phosphatase activity was assayed by the Reiner's method, a modification of the King-Armstrong method. The alkaline phosphatase activity of Placentaliquid was safety preserved at 0°C for 750 days, but the activity was almost declined at 30°C after 230 days.<br>Placentaliquid was tested for heat stability of the alkaline phosphase and tyrosinase inhibitory activities, Tyrosinase activity was assayed by colorimetric method, a modification of the Lerner's and Shima's method. As the results, 80% of the alkaline phosphatase activity decreased by at 60°C in 60 minutes, while 75% of the tyrosinase inhibitory activity was preserved at 60°C after 60 minutes.<br>It was considered that the heat inactivation of alkaline phosphatase was due to denaturation of the enzyme protein. On the other hand, the heat inactvation process of the tyrosinase inhibitory activity did not agree to that of the denaturation of the enzyme protein. So the activity of alkaline phosphatase was not closely related to the tyrosinase inhibitory activity. As the above results, it is hardly recognnized that the alkaline phosphatase activity is the most adequate indicator for the grading of Placentaliquid.

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390282679477470976
  • NII論文ID
    130004069646
  • DOI
    10.5107/sccj.13.52
  • COI
    1:CAS:528:DyaL3cXivVCmtw%3D%3D
  • ISSN
    18844146
    03875253
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

問題の指摘

ページトップへ