微生物を用いるｎｅｔｉｌｍｉｃｉｎの比色分析法　指示薬を含有するろ紙を用いる比色分析法

• 津田 良子

  シェリング・プラウ株式会社滋賀工場研究開発部

• 若松 浩美

  シェリング・プラウ株式会社滋賀工場研究開発部

• 藤本 尚

  シェリング・プラウ株式会社滋賀工場研究開発部

書誌事項

タイトル別名

• Studies on a colorimetric bioassay for netilmicin using an indicator disc.
• 指示薬を含有する濾紙を用いる比色分析法

抄録

A new colorimetric bioassay of an aminoglycoside antibiotic, netilmicin (NTL), was investigated. Discoloration of the color-indicator on the paper disc (indicator disc) is due to the presence of CO₂ produced by Bacillus subtilis. Discoloration of the indicator disc by CO₂ was studied as follows: A mixture of B. subtilis suspension, nutrient broth, 0.1 M phosphate buffer (pH 8.0) and NTL solution was transfered to a 5 ml Reacti-Vial^®. An indicator disc containing thymolphthalein (TP), methyl red (MR) and NaOH was attached to the inside of the Reacti-Vial septum. The vials were then placed in a shaking water-bath (37±1°C) for 3 h. The blue indicator disc was discolored, the degree of discoloration depending on the growth of B. subtilis in the medium. When the concentration of NTL was high and the level of CO₂ low, no change was observed. The color of the indicator disc was determined spectrophtometically after 3 h of incubation, using complementary tristimulus colorimetry, which is useful in determining pH-dependent chemical species like TP and MR. Our proposed method was found to save more than 13 h of working time when compared with the conventional bioassay method, so it is a useful and convenient method for determining NTL.

収録刊行物

• ＣＨＥＭＯＴＨＥＲＡＰＹ

  ＣＨＥＭＯＴＨＥＲＡＰＹ 41 (6), 641-648, 1993

  公益社団法人 日本化学療法学会

キーワード

• 比色分析
• CO<SUB>2</SUB>
• 酸・壇基指示薬
• コンプリメンタリートリスティミュラス法

詳細情報

• CRID: 1390282681255615872
• NII論文ID: 130004073033
• DOI: 10.11250/chemotherapy1953.41.641
• ISSN: 18845894
• ISSN: 00093165
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可