好熱性放線菌Theymoactinornyces vulgaris R-47からクローン化された二つのα-アミラーゼ,TVAIとTVAII,のグルコシルサイクロデキストリン分解に関する作用

DOI
  • 志村 洋一郎
    Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • 殿塚 隆史
    Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • 坂野 好幸
    Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology

書誌事項

タイトル別名
  • The Action of Two α-Amylases, TVA I and TVA II, from The rmoactinomyces vulgaris R-47 on Glucosyl Cyclodextrin

抄録

We performed a kinetic study to estimate the action mode of two α-amylases, TVA I and TVA II, on glucosyl cyclodextrins. The enzyme genes from Thermoactinomyces vulgaris R-47 were individually cloned and expressed in Escherichia coli, and the enzymes were prepared from the transformants. TVA I hydrolyzed glucosyl-γ-cyclodextrin well, whereas TVA II hydrolyzed glucosyl-α- and -β-cyclodextrins. TVA I and TVA II hydrolyzed glucosyl cyclodextrins to produce glucose, maltose, panose, and 62-α-glucosylmaltotriose.

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390282680147092224
  • NII論文ID
    130004091142
  • DOI
    10.11541/jag1994.45.393
  • COI
    1:CAS:528:DyaK1MXos1ynug%3D%3D
  • ISSN
    18844898
    13403494
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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