豚血清乳酸脱水素酵素の測定に関する検討  [in Japanese] Evaluation on results of assay of lactic dehydrogenase (LDH) in porcine serum.  [in Japanese]

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Abstract

豚の血清乳酸脱水素酵素 (以下, 血清LDH) の総活性値およびそのアイソザイムの測定値が検体の取扱い方法などの諸要因によりどのような影響を受けるのかについて調べ, あわせて肉豚の生理値についても検討し, 以下の結果を得た。<br>1. 血漿と血清の比較: 血漿 (ヘパリン処理) と血清のLDHの総活性値およびアイソザイムに差はなかった。<br>2. 放置時間が与える影響: 採血後2時間までの放置ではLDH<sub>2</sub>分画を除いて影響を受けなかった。<br>3. 血清の安定性: -80℃保存が最も安定で, 次いで室温, -20℃の順であり, 4℃保存は長期間では安定性を欠いた。<br>4. 溶血の影響: LDH<sub>2</sub>およびLDH<sub>3</sub>は溶血による影響をまったく受けず, 総活性値は廾より, LDH<sub>1</sub>は+, LDH<sub>4</sub>は柵卅, LDH<sub>5</sub>は廾より, それぞれ溶血による影響を受けた。<br>5. 生前血液と放血血液の比較: LDH総活性値およびアイソザイムのすべての項目について, 両者間に有意差はあったが, 有意な相関関係が認められた。<br>6. 肉豚の生理値: 生理値をm±2SDで示すと, LDH総活性値は1,270±448 Wróblewski 単位, LDH<sub>1</sub>は34.1±12.6%, LDH<sub>2</sub>は21.2±5.6%, LDH<sub>3</sub>は22.1±5.0%, LDH<sub>4</sub>は11.7±5.8%, LDH<sub>5</sub>は11.1±7.496であった。

It was investigated in the present study on what influences would be exerted on the total activity level of lactic dehydrogenase (abbr. as LDH) in porcine serum and the level of its isozyme by various factors such as the method for handling the test samples, along with evaluation of physiological values of fattening pigs. The following results were obtained.<br>1) Comparison of LDH activity level and its isozyme level in (heparinized) plasma and serum:<br>There were no significant differences between the levels of LDH activity and isozyme in (heparinized) plasma and serum.<br>2) Effect of leaving time from collecting blood till centrifugation upon the results of assay:<br>No appreciable effect was identified by the variation in the leaving time till 2 hours after collecting blood except for LDH<sub>2</sub> fraction.<br>3) Stability of LDH activity and its isozyme in serum: The stabilities were the highest when stored at -80°C followed by those at room temperature and those at -20°C in this decreasing order. Storage at 4°C proved a failure in maintaining the stability for a long period of time.<br>4) Effect of hemolysis upon assay results:<br>Neither LDH<sub>2</sub> nor LDH<sub>3</sub> received any influence upon their levels by the hemolysis, while perceivable influences upon assay results were initiated upon LDH activity from (_??_), and upon LDH<sub>1</sub>, LDH<sub>4</sub> and LDH<sub>5</sub> from (+), (_??_) and (_??_), respectively.<br>5) Comparison between the assay results of blood from living bodies (abbr. as living) and blood discharged by bleeding (abbr. as bleeding):<br>Significant differences were found out between them in terms of LDH activity and isozyme level. However, there were significant correlation between living and bleeding, and the regression equations were approved.<br>6) Physiological value of fattening pigs:<br>To demonsrate the physiological values with mean ±2SD. The activity of serum LDH was 1, 270±448 Wróblewski unit. LDH<sub>1</sub>, LDH<sub>2</sub>, LDH<sub>3</sub>, LDH<sub>4</sub> and LDH<sub>5</sub> were 34.1±12.6%, 21.2±5.6%, 22.1±5.0%, 11.7±5.8% and 11.1±7.4% respectively.

Journal

  • Nihon Yoton Gakkaishi

    Nihon Yoton Gakkaishi 25(1), 21-28, 1988

    The Japanese Society of Swine Science

Codes

  • NII Article ID (NAID)
    130004129027
  • Text Lang
    JPN
  • ISSN
    0913-882X
  • Data Source
    J-STAGE 
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