Implication of Amphiphysin 1 and Dynamin 2 in Tubulobulbar Complex Formation and Spermatid Release

• Kusumi Norihiro

  Department of Urology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Watanabe Masami

  Department of Urology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Yamada Hiroshi

  Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Li Shun-Ai

  Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Kashiwakura Yuji

  Center for Gene and Cell Therapy, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Matsukawa Takashi

  Department of Anesthesia, University of Yamanashi, Faculty of Medicine

• Nagai Atsushi

  Department of Urology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Nasu Yasutomo

  Department of Urology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Kumon Hiromi

  Department of Urology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Takei Kohji

  Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

抄録

Tubulobulbar complexes (TBCs) are composed of several tubular invaginations formed at the plasma membrane of testicular Sertoli cells. TBCs are transiently formed at the contact region with spermatids at spermatogenic stage VII in rat and mouse, and such TBC formation is prerequisite for spermatid release. Since the characteristic structure of TBCs suggests that the molecules implicated in endocytosis could be involved in TBC formation, we here investigated the localization and physiological roles of endocytic proteins, amphiphysin 1 and dynamin 2, at TBCs. We demonstrated by immunofluorescence that the endocytic proteins were concentrated at TBCs, where they colocalized with cytoskeletal proteins, such as actin and vinculin. Immunoelectron microscopy disclosed that both amphiphysin 1 and dynamin 2 were localized on TBC membrane. Next, we histologically examined the testis from amphiphysin 1 deficient {Amph^–/–} mice. Morphometric analysis revealed that the number of TBCs was significantly reduced in Amph^–/–. The ratio of stage VIII seminiferous tubules was increased, and the ratio of stage IX was conversely decreased in Amph^–/–. Moreover, unreleased spermatids in stage VIII seminiferous tubules were increased in Amph^–/–, indicating that spermatid release and the following transition from stage VIII to IX was prolonged in Amph^–/– mice. These results suggest that amphiphysin 1 and dynamin 2 are involved in TBC formation and spermatid release at Sertoli cells.<br>

収録刊行物

• Cell Structure and Function

  Cell Structure and Function 32 (2), 101-113, 2007

  日本細胞生物学会

キーワード

• amphiphysin 1
• dynamin 2
• tubulobulbar complex
• spermatogenesis
• testis
• endocytosis

詳細情報

• CRID: 1390282679670931200
• NII論文ID: 130004137522
• NII論文ID: 40016192023
• NII書誌ID: AA0060007X
• DOI: 10.1247/csf.07024
• ISSN: 13473700
• ISSN: 03867196
• NDL書誌ID: 9603584
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9603584
• Web Site: http://www.jstage.jst.go.jp/article/csf/32/2/32_2_101/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009003329
• 本文言語コード: en
• データソース種別:
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  • NDL
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