Endothelial Cells Constituting Blood-nerve Barrier Have Highly Specialized Characteristics as Barrier-forming Cells

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  • Sano Yasuteru
    Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
  • Shimizu Fumitaka
    Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
  • Nakayama Hiroto
    Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
  • Abe Masaaki
    Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
  • Maeda Toshihiko
    Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
  • Ohtsuki Sumio
    Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University
  • Terasaki Tetsuya
    Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University
  • Obinata Masuo
    Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University
  • Ueda Masatsugu
    The YS Institute, Inc.
  • Takahashi Ri-ichi
    The YS Institute, Inc.
  • Kanda Takashi
    Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine

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Abstract

In autoimmune disorders of the peripheral nervous system (PNS) such as Guillain-Barré syndrome and chronic inflammatory demyelinating polyradiculoneuropathy, breakdown of the blood-nerve barrier (BNB) has been considered as a key step in the disease process. Hence, it is important to know the cellular property of peripheral nerve microvascular endothelial cells (PnMECs) constituting the bulk of BNB. Although many in vitro models of the blood-brain barrier (BBB) have been established, very few in vitro BNB models have been reported so far. We isolated PnMECs from transgenic rats harboring the temperature-sensitive SV40 large T-antigen gene (tsA58 rat) and investigated the properties of these “barrier-forming cells”. Isolated PnMECs (TR-BNBs) showed high transendothelial electrical resistance and expressed tight junction components and various types of influx as well as efflux transporters that have been reported to function at BBB. Furthermore, we confirmed the in vivo expression of various BBB-forming endothelial cell markers in the endoneurium of a rat sciatic nerve. These results suggest that PnMECs constituting the bulk of BNB have a highly specialized characteristic resembling the endothelial cells forming BBB.<br>

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