Mechanism Regulating Ca2+-dependent Mechanosensory Behaviour in Sea Urchin Spermatozoa

• Kambara Yuka

  Department of Biological Sciences, Graduate School of Science, University of Tokyo

• Shiba Kogiku

  Misaki Marine Biological Station, Graduate School of Science, University of Tokyo

• Yoshida Manabu

  Misaki Marine Biological Station, Graduate School of Science, University of Tokyo

• Sato Chihiro

  Bioscience and Biotechnology Center, Nagoya University

• Kitajima Ken

  Bioscience and Biotechnology Center, Nagoya University

• Shingyoji Chikako

  Department of Biological Sciences, Graduate School of Science, University of Tokyo

Bibliographic Information

Other Title

• Mechanisms regulating Ca2+-dependent mechanosensory behaviour in sea urchin spermatozoa

Abstract

Flagellar movement of the sea urchin sperm is regulated by intracellular Ca²⁺. Flagellasialin, a polysialic acid-containing glycoprotein, as well as other membrane proteins seems responsible for the Ca²⁺ control. To elucidate the mechanism of Ca²⁺ dynamics underlying flagellar movement, we analysed the sperm’s mechanosensory behavioural responses by using microtechniques. In sea water containing 10 mM Ca²⁺, the sperm swim in circular paths. When a mechanical stimulus was applied to the sperm head with a glass microstylus, the sperm showed a series of flagellar responses, consisting of a stoppage of beating (quiescence) and a recovery of swimming in a straight path, followed by swimming in a circular path again; as the result the sperm avoided the obstacle. Ca²⁺-imaging with Fluo-4 showed that the intracellular Ca²⁺ was high in the quiescence and gradually decreased after that. The effects of blockers and antibodies against candidate components revealed that the Ca²⁺ influx was induced by Ca²⁺ channels and the Ca²⁺ efflux was induced by a flagellasialin-related Ca²⁺-efflux system, plasma membrane Ca²⁺-ATPases and the K⁺-dependent Na⁺/Ca²⁺ exchanger. The results show that the Ca²⁺-dependent mechanosensory behaviour of the sea urchin sperm is regulated by organized functioning of the membrane environment including the plasma membrane proteins and flagellasialin.<br>

Journal

• Cell Structure and Function

  Cell Structure and Function 36 (1), 69-82, 2011

  Japan Society for Cell Biology

Keywords

• avoiding response
• calcium
• flagellasialin
• plasma membrane Ca²⁺-ATPase
• K⁺-dependent Na⁺/Ca²⁺ exchanger

Details

• CRID: 1390001204695188992
• NII Article ID: 130004137565
• NII Article ID: 40019163920
• NII Book ID: AA0060007X
• DOI: 10.1247/csf.10020
• ISSN: 13473700
• ISSN: 03867196
• ISSN: http://id.crossref.org/issn/03867196
• NDL BIB ID: 023442801
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023442801
• Web Site: http://www.jstage.jst.go.jp/article/csf/36/1/36_10020/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2013092014
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed