Temporal and Spatial Pattern of <i>Dref</i> Expression during <i>Drosophila</i> Bristle Development

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Author(s)

    • Kawamori Akihito
    • Department of Applied Biology, Kyoto Institute of Technology|Insect Biomedical Research Center, Kyoto Institute of Technology
    • Shimaji Kouhei
    • Department of Applied Biology, Kyoto Institute of Technology|Insect Biomedical Research Center, Kyoto Institute of Technology
    • Yamaguchi Masamitsu
    • Department of Applied Biology, Kyoto Institute of Technology|Insect Biomedical Research Center, Kyoto Institute of Technology

Abstract

The DNA replication-related element-binding factor (DREF) is a BED finger-type transcription factor that has important roles in cell cycle progression. In an earlier study, we showed that DREF is required for endoreplication during posterior scutellar macrochaete development. However, dynamic change in the <i>dref</i> expression in the cell lineage is unclear. In this study, we focused on the spatio-temporal pattern of expression of the <i>dref</i> gene during bristle development. Gene expression analysis using GAL4 enhancer trap lines of <i>dref</i> and the upstream activation sequence-green fluorescent protein with nuclear localization signals (UAS-GFPnls) in combination with immunostaining revealed the half-life of GFPnls <i>in vivo</i> (<6 hours) is short enough to monitor the <i>dref</i> gene expression. The analysis revealed that the <i>dref</i> expression occurs in clusters that include cells consisting of a bristle as well as surrounding epidermal cells. The intensity of GFP signals was almost the same in those cells, suggesting expression of the <i>dref</i> gene in bristle cell lineages occurs simultaneously in clusters. Further analysis showed that GFP signals increased twice during sensory organ precursor development as well as in bristle development at 9 hours and 15 hours after pupal formation, respectively. However, its expression was barely detectable in the cell lineages in and around asymmetric cell division or at other stages of development. For the first time, we clarified a spatio-temporal pattern of expression of the <i>dref</i> gene <i>in vivo</i> and revealed that expression of the <i>dref</i> gene occurs in clusters and is temporally regulated at specific times during bristle development.

Journal

  • Cell Structure and Function

    Cell Structure and Function 38(2), 169-181, 2013

    Japan Society for Cell Biology

Codes

  • NII Article ID (NAID)
    130004137594
  • Text Lang
    ENG
  • ISSN
    0386-7196
  • Data Source
    J-STAGE 
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