KISS1 Gene Expression in the Developing Brain of Female Pigs in Pre- and Peripubertal Periods

• IEDA Nahoko

  Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

• UENOYAMA Yoshihisa

  Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

• TAJIMA Yoko

  Aichi Agricultural Research Center, Nagakute 480-1193, Japan

• NAKATA Tomoko

  Aichi Agricultural Research Center, Nagakute 480-1193, Japan

• KANO Masatoshi

  Aichi Agricultural Research Center, Nagakute 480-1193, Japan

• NANIWA Yousuke

  Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

• WATANABE Youki

  Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

• MINABE Shiori

  Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

• TOMIKAWA Junko

  Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

• INOUE Naoko

  Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

• MATSUDA Fuko

  Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

• OHKURA Satoshi

  Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

• MAEDA Kei-ichiro

  Department of Veterinary Medical Sciences, The University of Tokyo, Tokyo 113-8657, Japan

• TSUKAMURA Hiroko

  Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

書誌事項

タイトル別名

• <i>KISS1</i> Gene Expression in the Developing Brain of Female Pigs in Pre- and Peripubertal Periods

抄録

Puberty is associated with an increase in gonadotropin secretion as a result of an increase in gonadotropin-releasing hormone (GnRH) secretion. Kisspeptin is considered to play a key role in puberty onset in many mammalian species, including rodents, ruminants and primates. The present study aimed to determine if changes in hypothalamic expression of the KISS1 gene, encoding kisspeptin, are associated with the onset of puberty in pigs. The animals (n=4 in each group) were perfused with 4% paraformaldehyde at 0, 1, 2, 3 and 4 months old, as prepubertal stages, and at 5 months old, as the peripubertal stage, following each blood sampling. KISS1 gene expressions in coronal sections of brains were visualized by in situ hybridization. Plasma luteinizing hormone (LH) was measured by radioimmunoassay. KISS1 mRNA signals were observed in the arcuate nucleus (ARC) at all ages examined without any significant difference in the number of KISS1-expressing cells, indicating that the KISS1 gene is constantly expressed in the ARC throughout pubertal development in pigs. The plasma LH concentration was the highest in 0-month-old piglets and significantly decreased in the 1- and 2 month-old groups (P<0.05), suggesting a developing negative feedback mechanism affecting gonadotropin release during the prepubertal period. Considering the potent stimulating effect of kisspeptin on gonadotropin release in prepubertal pigs, kisspeptin secretion rather than kisspeptin synthesis may be responsible for the onset of puberty in pigs.

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 60 (4), 312-316, 2014

  公益社団法人 日本繁殖生物学会

キーワード

• Estradiol
• GPR54
• Kisspeptin
• Porcine
• Progesterone

詳細情報

• CRID: 1390001206337689728
• NII論文ID: 130004137827
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.2013-129
• COI: 1:STN:280:DC%2BC2cfgtlWquw%3D%3D
• ISSN: 13484400
• ISSN: 09168818
• NDL書誌ID: 025629542
• PubMed: 24909600
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025629542
• Web Site: https://www.jstage.jst.go.jp/article/jrd/60/4/60_2013-129/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015062389
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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