Expression and Localization of α-amylase in the Submandibular and Sublingual Glands of Mice

• Yamagishi Ryoko

  Department of Histology and Embryology, Graduate School of Medical Sciences, Kanazawa University

• Wakayama Tomohiko

  Department of Histology and Embryology, Graduate School of Medical Sciences, Kanazawa University

• Nakata Hiroki

  Department of Histology and Embryology, Graduate School of Medical Sciences, Kanazawa University

• Adthapanyawanich Kannika

  Department of Histology and Embryology, Graduate School of Medical Sciences, Kanazawa University

• Kumchantuek Tewarat

  Department of Histology and Embryology, Graduate School of Medical Sciences, Kanazawa University

• Yamamoto Miyuki

  Department of Histology and Embryology, Graduate School of Medical Sciences, Kanazawa University

• Iseki Shoichi

  Department of Histology and Embryology, Graduate School of Medical Sciences, Kanazawa University

抄録

In the major salivary glands of mice, acinar cells in the parotid gland (PG) are known to be the main site for the production of the digestive enzyme α-amylase, whereas α-amylase production in the submandibular gland (SMG) and sublingual gland (SLG), as well as the cell types responsible for α-amylase production, has been less firmly established. To clarify this issue, we examined the expression and localization of both the mRNA and protein of α-amylase in the major salivary glands of male and female mice by quantitative and histochemical methods. α-amylase mRNA levels were higher in the order of PG, SMG, and SLG. No sexual difference was observed in α-amylase mRNA levels in the PG and SLG, whereas α-amylase mRNA levels in the female SMG were approximately 30% those in the male SMG. Using in situ hybridization and immunohistochemistry, signals for α-amylase mRNA and protein were found to be strongly positive in acinar cells of the PG, serous demilune cells of the SLG, and granular convoluted tubule (GCT) cells of the male SMG, weakly positive in seromucous acinar cells of the male and female SMG, and negative in mucous acinar cells of the SLG. These results clarified that α-amylase is produced mainly by GCT cells and partly by acinar cells in the SMG, whereas it is produced exclusively by serous demilune cells in the SLG of mice.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 47 (3), 95-102, 2014

  日本組織細胞化学会

キーワード

• α-amylase
• salivary gland
• acinus
• duct
• sexual dimorphism
• mouse

詳細情報

• CRID: 1390282679839413888
• NII論文ID: 130004137835
• NII書誌ID: AA11504078
• DOI: 10.1267/ahc.14005
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://hdl.handle.net/2297/39719
• Web Site: https://www.jstage.jst.go.jp/article/ahc/47/3/47_14005/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015062266
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可