BOB.1-positive Classical Hodgkin's Lymphoma Carries Hypermethylation of Its Promoter as Epigenetic Marker of Gene-silencing Memory

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Author(s)

    • Watanabe Takafumi
    • Department of Molecular Pathology, Ehime University Graduate School of Medicine
    • Kitazawa Riko
    • Department of Molecular Pathology, Ehime University Graduate School of Medicine
    • Mizuno Yosuke
    • Department of Molecular Pathology, Ehime University Graduate School of Medicine
    • Kuwahara Natsumi
    • Department of Molecular Pathology, Ehime University Graduate School of Medicine
    • Ito Chizu
    • Department of Molecular Pathology, Ehime University Graduate School of Medicine
    • Sugita Atsuro
    • Department of Molecular Pathology, Ehime University Graduate School of Medicine
    • Haraguchi Ryuma
    • Department of Molecular Pathology, Ehime University Graduate School of Medicine
    • Kitazawa Sohei
    • Department of Molecular Pathology, Ehime University Graduate School of Medicine

Abstract

Analysis of archival formalin-fixed, paraffin-embedded (FFPE) pathological specimens of three case of Epstein-Barr virus (EBV)-positive diffuse large B-cell lymphoma (DLBCL) and three cases of classical Hodgkin lymphoma (CHL) revealed that hypermethylation of the <i>BOB.1</i> gene promoter was exclusively observed in CHL. A discrepancy was observed, however, between the methylation status of the <i>BOB.1</i> gene promoter and its expression in the EBV-positive mixed cellular CHL (MCCHL). Since MCCHL lacks the typical B-cell phenotype even in the presence of abundant BOB.1 transcription factors, functional activity of BOB.1 may be lost or reduced by a mechanism other than epigenetic gene silencing. When some tumor-suppressor gene products have lost their biological function, impact or significance of derepression of such genes may be little. Therefore, when interpreting immunohistochemical results for diagnostic or research purposes, it must be borne in mind that apparent positive immunostaining can merely be the result of chromatin remodeling and that such transient expression often has little functional significance. Any apparent positive immunohistochemical result needs to be interpreted carefully with the help of the hypermethylation status as a molecular marker of gene silencing memory.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 47(3), 125-131, 2014

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

Codes

  • NII Article ID (NAID)
    130004137836
  • Text Lang
    ENG
  • ISSN
    0044-5991
  • Data Source
    J-STAGE 
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