The Involvement of L-Type Amino Acid Transporters in Theanine Transport

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  • YAMAMOTO Sachiko
    Department of Chemistry, Kyorin University School of Medicine
  • KIMURA Toru
    Department of Pharmacology and Toxicology, Kyorin University School of Medicine
  • TACHIKI Takashi
    Department of Biotechnology, Faculty of Life Science, Ritsumeikan University
  • ANZAI Naohiko
    Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine
  • SAKURAI Takuya
    Department of Molecular Predictive Medicine and Sport Science, Kyorin University School of Medicine
  • USHIMARU Makoto
    Department of Chemistry, Kyorin University School of Medicine

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L-Theanine has favorable physiological effects in terms of human health, but the mechanisms that transport it to its target organs or cells are not completely defined. To identify the major transport mechanisms of L-theanine, we screened for candidate transporters of L-3H-theanine in several mammal cell lines that intrinsically express multiple transporters with various specificities. All of the cells tested, T24, HepG2, COS1, 293A, Neuro2a, and HuH7, absorbed L-3H-theanine. Uptake was significantly inhibited by the addition of L-leucine and by a specific inhibitor of the system L transport system, 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH). L-3H-Theanine uptake occurred mostly independently of Na+. These results indicate that L-theanine was taken up via a system L like transport system in all of the cells tested. Additionally, in experiments using cells stably expressing two system L isoforms, LAT1 and LAT2, we found that the two isoforms mediated L-theanine transport to similar extents. Taken together, our results indicate that L-theanine is transported mostly via the system L transport pathway and its isoforms.

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