Enzymatic and Molecular Characterization of Arabidopsis ppGpp Pyrophosphohydrolase, AtNUDX26
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- ITO Daisuke
- Department of Advanced Bioscience, Faculty of Agriculture, Kinki University
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- KATO Takahiro
- Department of Advanced Bioscience, Faculty of Agriculture, Kinki University
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- MARUTA Takanori
- Faculty of Life and Environmental Science, Shimane University Department of Advanced Bioscience, Faculty of Agriculture, Kinki University
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- TAMOI Masahiro
- Department of Advanced Bioscience, Faculty of Agriculture, Kinki University
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- YOSHIMURA Kazuya
- Department of Food and Nutritional Science, College of Bioscience and Biotechnology, Chubu University
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- SHIGEOKA Shigeru
- Department of Advanced Bioscience, Faculty of Agriculture, Kinki University
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Not only in bacteria but also in plant cells, guanosine-3′,5′-tetraphosphate (ppGpp) is an important signaling molecule, that affects various cellular processes. In this study, we identified nucleoside diphosphates linked to some moiety X (Nudix) hydrolases, AtNUDX11, 15, 25, and 26, having ppGpp pyrophosphohydrolase activity from Arabidopsis plants. Among these, AtNUDX26 localized in chloroplasts had the highest Vmax and kcat values, leading to high catalytic efficiency, kcat/Km. The activity of AtNUDX26 required Mg2+ or Mn2+ ions as cofactor and was optimal at pH 9.0 and 50 °C. The expression of AtNUDX26 and of ppGpp metabolism-associated genes was regulated by various types of stress, suggesting that AtNUDX26 regulates cellular ppGpp levels in response to stress and impacts gene expression in chloroplasts. This is the first report on the molecular properties of ppGpp pyrophosphohydrolases in plants.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 76 (12), 2236-2241, 2012
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206479998336
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- NII論文ID
- 130004137889
- 10031146838
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3s7pvVSrtg%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 024165258
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- PubMed
- 23221701
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可