An Adhesin-Like Protein, Lam29, from <i>Lactobacillus mucosae</i> ME-340 Binds to Histone H3 and Blood Group Antigens in Human Colonic Mucus

• WATANABE Masamichi

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• KINOSHITA Hideki

  School of Food, Agricultural and Environmental Sciences, Miyagi University School of Food, Agricultural and Environmental Sciences, Miyagi University

• HUANG I-Nung

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• EGUCHI Kei

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• TSURUMI Takuya

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• KAWAI Yasushi

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• KITAZAWA Haruki

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• KIMURA Katsunori

  Division of Research and Development, Meiji Dairies Corporation Division of Research and Development, Meiji Dairies Corporation

• TAKETOMO Naoki

  Division of Research and Development, Meiji Dairies Corporation Division of Research and Development, Meiji Dairies Corporation

• KIKUCHI Daisuke

  Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine

• SASE Tomohiko

  Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine

• MIURA Koh

  Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine

• OGAWA Hitoshi

  Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine

• SHIBATA Chikashi

  Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine Division of Biological Regulation and Oncology, Department of Surgery, Tohoku University Graduate School of Medicine

• HORII Akira

  Department of Molecular Pathology, Tohoku University School of Medicine Department of Molecular Pathology, Tohoku University School of Medicine

• SAITO Tadao

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

Bibliographic Information

Other Title

• An Adhesin-Like Protein, Lam29, from Lactobacillus mucosae ME-340 Binds to Histone H3 and Blood Group Antigens in Human Colonic Mucus

Abstract

A cell-surface 29-kDa protein (Lam29, cysteine-binding protein of the ABC transporter) from Lactobacillus mucosae ME-340 showed an adhesin-like property for human ABO blood group antigens expressed on the gastrointestinal mucosa. In addition, here we report that Lam29 also bound to an 18-kDa protein on human colonic mucus. By ligand blot assay and N-terminal amino acid sequence of the protein, it was identified as human histone H3. By ligand blot and microplate binding assays with recombinant histone H3, binding between Lam29 and histone H3 was confirmed. The adhesion of ME-340 cells to histone H3 was significantly inhibited by 26% after the addition of 2.5 mg/mL Lam29 as compared to the absence of Lam29 (p<0.01). By GHCl extraction and transcription attenuation of ME-340 cells, binding reduction of ME340 cells against histone H3 was detected at 12% and 13% respectively, as compared to control cells by the BIACORE assay (p<0.01). These data indicate that Lam29 shows multiple binding activities to blood group antigens and histone H3 in human colonic mucus. This is the first report to indicate that lactobacilli expressing Lam29 adhere to histone H3 on gastrointestinal mucosa.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 76 (9), 1655-1660, 2012

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• adhesion
• human colonic mucus
• histone H3
• ABC transporter
• <i>lactobacillus</i>

Details

• CRID: 1390282681454652288
• NII Article ID: 130004137955
• NII Article ID: 10031123168
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.120150
• COI: 1:STN:280:DC%2BC38bmsVWiug%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 023966122
• PubMed: 22972326
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023966122
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.120150
• Web Site: https://search.jamas.or.jp/link/ui/2013124399
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed