CoCl2 Inhibits Neural Differentiation of Retinoic Acid-Treated Embryoid Bodies

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Author(s)

Abstract

The effects of CoCl<sub>2</sub> on retinoic acid (RA)-treated embryoid bodies (EBs) were investigated. Four-day EBs were treated with 5×10<sup>–6</sup> M of RA for 4 d, then subjected to attached culturing for 7 d in the presence of CoCl<sub>2</sub> at 0, 20, and 100 μM. Differentiation into MAP2- and GFAP-immunopositive cells was inhibited by CoCl<sub>2</sub> in a dose-dependent manner. Next, RA-treated EBs were dissociated into single cells and cultured for 7 d at an initial cell density of 1×10<sup>3</sup>/cm<sup>2</sup>. The number of cells increased in a CoCl<sub>2</sub>-dose dependent fashion. In cultures with 100 μM of CoCl<sub>2</sub>, more than 90% of the cells were immunopositive for nestin and nestin-immunopositive cells formed clusters, while there were few cells immunopositive for MAP2 or GFAP. These results suggest that CoCl<sub>2</sub> inhibits neural differentiation of RA-treated EB cells and promotes the proliferation of nestin-immunopositive cells, <i>i.e.</i>, embryonic stem (ES)-derived neural stem-like cells.<br>

Journal

  • Journal of Bioscience and Bioengineering

    Journal of Bioscience and Bioengineering 106(2), 141-147, 2008

    The Society for Biotechnology, Japan

Codes

  • NII Article ID (NAID)
    130004144994
  • Text Lang
    ENG
  • ISSN
    1389-1723
  • Data Source
    J-STAGE 
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