Single-Molecule Reverse Transcription Polymerase Chain Reaction Using Water-in-Oil Emulsion

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Author(s)

    • NAKANO MICHIHIKO
    • Department of Ecological Engineering, Toyohashi University of Technology
    • NAKAI NAOHITO
    • Department of Ecological Engineering, Toyohashi University of Technology
    • KURITA HIROFUMI
    • Department of Ecological Engineering, Toyohashi University of Technology
    • KOMATSU JUN
    • Department of Ecological Engineering, Toyohashi University of Technology
    • KATSURA SHINJI
    • Department of Ecological Engineering, Toyohashi University of Technology
    • MIZUNO AKIRA
    • Department of Ecological Engineering, Toyohashi University of Technology

Abstract

A new method based on a combination of reverse transcription polymerase chain reaction (RT-PCR) and a water-in-oil (W/O) emulsion was developed. Reverse transcription and initial thermal cycles were carried out in droplets of the W/O emulsion. Then, the droplets were united, followed by remaining secondary PCR cycles. This two-step method succeeded in detecting a single RNA molecule.<br>

Journal

  • Journal of Bioscience and Bioengineering

    Journal of Bioscience and Bioengineering 99(3), 293-295, 2005

    The Society for Biotechnology, Japan

Codes

  • NII Article ID (NAID)
    130004145762
  • Text Lang
    ENG
  • ISSN
    1389-1723
  • Data Source
    J-STAGE 
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