Novel GmFAD2-1b mutant alleles created by reverse genetics induce marked elevation of oleic acid content in soybean seeds in combination with GmFAD2-1a mutant alleles

• Hoshino Tomoki

  Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Saga University

• Takagi Yutaka

  Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Saga University

• Anai Toyoaki

  Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Saga University

Abstract

The generation of useful mutant alleles of specific genes would accelerate conventional breeding programs in various commercially important crops. Common soybean oil is easily oxidized because it is rich in polyunsaturated fatty acids (PUFAs). Microsomal omega-6 fatty acid desaturase (FAD2), which introduces a second unsaturated bond into oleic acid, is a primary target for elevating oleic acid levels and reducing PUFA levels. The paleopolyploid soybean genome contains five FAD2 gene homologues, at least three of which (GmFAD2-1a, 2-1b, and 2-2a) are functional. In spite of their importance, very little genetic variation has been identified in these genes except in GmFAD2-1a, because fatty acid content is easily affected by environmental conditions such as temperature. Here we isolated novel mutant alleles of GmFAD2-1b from ethyl methanesulfonate-treated soybean mutant populations through Targeting Induced Local Lesions In Genomes (TILLING), a reverse genetic method. Evaluation of enzyme activity in a yeast heterologous expression system suggested that two mutant lines, ‘B12’ and ‘E11’, contain near-null and null alleles, respectively, of GmFAD2-1b. Furthermore, by combining GmFAD2-1a and GmFAD2-1b mutant alleles, we successfully generated soybean lines with >80% oleic acid content. TILLING could provide a practical method for expanding the genetic diversity of polyploid crops.<br>

Journal

• Breeding Science

  Breeding Science 60 (4), 419-425, 2010

  Japanese Society of Breeding

Keywords

• TILLING
• reverse genetics
• mutant library
• <i>FAD2</i>
• high oleic acid
• <i>Glycine max</i>
• marker-assisted selection

Details

• CRID: 1390001204722249600
• NII Article ID: 130004146252
• NII Article ID: 10027120468
• NII Book ID: AA11317194
• DOI: 10.1270/jsbbs.60.419
• COI: 1:CAS:528:DC%2BC3MXktFWltrs%3D
• ISSN: 13473735
• ISSN: 13447610
• NDL BIB ID: 10925675
• Web Site: http://id.ndl.go.jp/bib/10925675
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10925675
• Web Site: http://www.jstage.jst.go.jp/article/jsbbs/60/4/60_4_419/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed