Reverted glutathione <i>S</i>-transferase-like genes that influence flower color intensity of carnation (<i>Dianthus caryophyllus</i> L.) originated from excision of a transposable element

• Momose Masaki

  Central Laboratories for Key Technologies, Kirin Co., Ltd.

• Itoh Yoshio

  Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology deceased

• Umemoto Naoyuki

  Central Laboratories for Key Technologies, Kirin Co., Ltd.

• Nakayama Masayoshi

  Institute of Floricultural Science, National Agriculture and Food Research Organization

• Ozeki Yoshihiro

  Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology

Bibliographic Information

Other Title

• Reverted glutathione S-transferase-like genes that influence flower color intensity of carnation (Dianthus caryophyllus L.) originated from excision of a transposable element

Abstract

A glutathione S-transferase-like gene, DcGSTF2, is responsible for carnation (Dianthus caryophyllus L.) flower color intensity. Two defective genes, DcGSTF2mu with a nonsense mutation and DcGSTF2-dTac1 containing a transposable element dTac1, have been characterized in detail in this report. dTac1 is an active element that produces reverted functional genes by excision of the element. A pale-pink cultivar ‘Daisy’ carries both defective genes, whereas a spontaneous deep-colored mutant ‘Daisy-VPR’ lost the element from DcGSTF2-dTac1. This finding confirmed that dTac1 is active and that the resulting reverted gene, DcGSTF2rev1, missing the element is responsible for this color change. Crosses between the pale-colored cultivar ‘06-LA’ and a deep-colored cultivar ‘Spectrum’ produced segregating progeny. Only the deep-colored progeny had DcGSTF2rev2 derived from the ‘Spectrum’ parent, whereas progeny with pale-colored flowers had defective forms from both parents, DcGSTF2mu and DcGSTF2-dTac1. Thus, DcGSTF2rev2 had functional activity and likely originated from excision of dTac1 since there was a footprint sequence at the vacated site of the dTac1 insertion. Characterizing the DcGSTF2 genes in several cultivars revealed that the two functional genes, DcGSTF2rev1 and DcGSTF2rev2, have been used for some time in carnation breeding with the latter in use for more than half a century.

Journal

• Breeding Science

  Breeding Science 63 (4), 435-440, 2013

  Japanese Society of Breeding

Keywords

• anthocyanin
• CACTA
• <i>Dianthus</i>
• glutathione <i>S</i>-transferase
• <i>S</i> locus
• transposable element

Details

• CRID: 1390282679699193728
• NII Article ID: 130004146407
• NII Article ID: 40019944849
• NII Book ID: AA11317194
• DOI: 10.1270/jsbbs.63.435
• COI: 1:STN:280:DC%2BC2czivVKkug%3D%3D
• ISSN: 13473735
• ISSN: 13447610
• NDL BIB ID: 025150614
• PubMed: 24399917
• Web Site: http://id.ndl.go.jp/bib/025150614
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025150614
• Web Site: https://www.jstage.jst.go.jp/article/jsbbs/63/4/63_435/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed