Chondrogenic and Osteogenic Differentiation of Adipose-derived Stem Cells Isolated from GFP Transgenic Mice

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Author(s)

    • Ogawa Rei
    • Department of Plastic and Reconstructive Surgery, Nippon Medical School
    • Mizuno Hiroshi
    • Department of Plastic and Reconstructive Surgery, Nippon Medical School
    • Hyakusoku Hiko
    • Department of Plastic and Reconstructive Surgery, Nippon Medical School
    • Watanabe Atsushi
    • Department of Biochemistry and Molecular Biology, Nippon Medical School
    • Migita Makoto
    • Department of Biochemistry and Molecular Biology, Nippon Medical School
    • Shimada Takashi
    • Department of Biochemistry and Molecular Biology, Nippon Medical School

Abstract

Recent studies suggest that human adipose tissue contain pluripotent cells similar to bone marrow-derived stromal cells (BSCs)<sup>1-3</sup>. Taking advantage of homogeneously marked cells from green fluorescent protein (GFP) transgenic mice<sup>4</sup>, we have previously demonstrated that BSCs differentiate into a variety of cell lineages both <i>in vitro</i> and <i>in vivo</i><sup>5</sup>. In the present study, we extend this approach to characterize adipose-derived stromal cells (ASCs)<sup>6</sup>. These cells derived from human are sometimes called processed lipoaspirate (PLA) cells. ASCs were prepared from inguinal fat pads of GFP transgenic mice after extensive washing with PBS and treatment with collagenase. After the primary culture in control medium (DMEM+10%FBS), the cells were incubated in either chondrogenic medium (DMEM+1%FBS+insulin+ascorbate 2-phosphate+TGF-beta 1) or osteogenic medium (DMEM+10%FBS+dexamethasone+ascorbate-2-phosphate+beta-glycerophosphate) for two to four weeks. Chondrogenic differentiation was assessed by Alcian blue staining, while osteogenic differentiation was by von Kossa and Alkaline phosphatase staining. ASCs incubated in chondrogenic medium induced Alcian blue positive cells. Incubation with osteogenic medium became positive for von Kossa and Alkaline phosphatase staining. No osteochondrogenic differentiation was observed in cells incubated with control medium. This cell population can be easily identified through fluorescence microscope, it should be an ideal source of ASCs for further experiments of stem cell biology and tissue engineering.<br>

Journal

  • Journal of Nippon Medical School

    Journal of Nippon Medical School 71(4), 240-241, 2004

    The Medical Association of Nippon Medical School

Codes

  • NII Article ID (NAID)
    130004146741
  • Text Lang
    ENG
  • ISSN
    1345-4676
  • Data Source
    J-STAGE 
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