Surgical Technique of Orthotopic Liver Transplantation in Rats: The Kamada Technique and a New Splint Technique for Hepatic Artery Reconstruction
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- Ishii Eiichi
- Department of Pathology (Analytic Human Pathology), Nippon Medical School
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- Shimizu Akira
- Department of Pathology (Analytic Human Pathology), Nippon Medical School
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- Takahashi Mikiko
- Department of Pathology (Analytic Human Pathology), Nippon Medical School
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- Terasaki Mika
- Department of Pathology (Analytic Human Pathology), Nippon Medical School
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- Kunugi Shinobu
- Department of Pathology (Analytic Human Pathology), Nippon Medical School
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- Nagasaka Shinya
- Department of Pathology (Analytic Human Pathology), Nippon Medical School
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- Terasaki Yasuhiro
- Department of Pathology (Analytic Human Pathology), Nippon Medical School
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- Ohashi Ryuji
- Division of Diagnostic Pathology, Nippon Medical School Hospital
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- Masuda Yukinari
- Department of Pathology (Analytic Human Pathology), Nippon Medical School
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- Fukuda Yuh
- Department of Pathology (Analytic Human Pathology), Nippon Medical School
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抄録
Orthotopic liver transplantation (OLT) in rats is technically feasible and useful for the assessment of clinical liver transplantation and analysis of inflammatory liver diseases. OLT in rats was pioneered by Lee et al. in 1973 using hand-suture techniques of all vessels. This model has not been widely used due to the long operative time and technical demand. The cuff method was introduced by Kamada in 1979, and today, the Kamada technique is the one most commonly used worldwide. However, this technique does not include hepatic artery reconstruction, although this procedure is routinely performed in clinical transplantation. Nevertheless, several techniques for hepatic artery reconstruction in rat OLT have been reported recently, and our group also developed a simple splint technique from recipient right renal artery to donor celiac axis bearing the hepatic artery. In the present article, we describe the Kamada technique, as a standard surgical method for rat OLT. In addition, we also describe our splint technique for hepatic artery reconstruction. Then, we compare the features of Kamada technique and our splint technique for hepatic artery reconstruction and all other surgical techniques currently in use for rat OLT. The widespread use of the rat OLT model should help to provide full assessment of transplant immunology and the mechanism and treatment of inflammatory liver diseases.<br>
収録刊行物
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- 日医大誌
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日医大誌 80 (1), 4-15, 2013
日本医科大学医学会