Differential Expression of ADAM (a Disintegrin and Metalloproteinase) Genes between Human First Trimester Villous and Extravillous Trophoblast Cells

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    • Takahashi Hironori
    • Department of Obstetrics and Gynecology, Jichi Medical University|Department of Molecular Medicine and Anatomy, Nippon Medical School
    • Yuge Kazuya
    • Department of Molecular Medicine and Anatomy, Nippon Medical School
    • Usui Rie
    • Department of Obstetrics and Gynecology, Jichi Medical University


A disintegrin and metalloproteinases (ADAMs) are members of the metzincin family of zinc-dependent metalloproteinases that play pivotal roles in the proteolytic degradation of the extracellular matrix for cell invasion. Few studies have investigated the ADAM subtypes that are expressed in first trimester trophoblast cells. The purpose of this study was to elucidate the differential expression profiles of ADAMs between first trimester villous trophoblast cells (VTs) and extravillous trophoblast cells (EVTs). We isolated EVTs from explanted human first trimester chorionic villi and investigated the mRNA expression levels of five members of the ADAM family (<i>ADAMTS1</i>, <i>ADAMTS2</i>, <i>ADAM10</i>, <i>ADAM12</i>, and <i>ADAM17</i>) using real-time PCR. Chorionic villous tips were defined as first trimester VTs. Of the differentially expressed ADAM genes between first trimester VTs and EVTs, <i>ADAMTS1</i> was expressed at a significantly higher level in EVTs than in VTs. In contrast, both <i>ADAM10</i> and <i>ADAM12</i> were expressed at significantly higher levels in VTs than in EVTs. No differences were found in the mRNA levels of <i>ADAMTS2</i> and <i>ADAM17</i> between the two cell types. Moreover, we demonstrated that in VTs, the expression level of <i>ADAM12</i> was significantly downregulated in the late first trimester (10-13 gestational weeks) compared to the middle first trimester (7-8 weeks). These results suggest that first trimester trophoblast cells express ADAM genes in cell type- and gestational age-dependent manners. Our data provide additional insight into the functions of ADAMs in the human placenta.<br>


  • Journal of Nippon Medical School

    Journal of Nippon Medical School 81(3), 122-129, 2014

    The Medical Association of Nippon Medical School


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