A Novel Transgenic Mouse Model Carrying Human Tribbles Related Protein 3 (TRB3) Gene and Its Site Specific Phenotype

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Author(s)

    • Sakai Yuto Sakai Yuto
    • Department of Drug Metabolism and Disposition, Graduate School of Pharmaceutical Sciences, Nagoya City University|Department of Molecular Toxicology, Graduate School of Medical Sciences and Medical School, Nagoya City University
    • Miyoshi Ichiro
    • Department of Comparative and Experimental Medicine, Graduate School of Medical Sciences and Medical School, Nagoya City University
    • Suzui Masumi
    • Department of Molecular Toxicology, Graduate School of Medical Sciences and Medical School, Nagoya City University
    • Hayashi Hidetoshi
    • Department of Drug Metabolism and Disposition, Graduate School of Pharmaceutical Sciences, Nagoya City University

Abstract

Tribbles related protein 3 (TRB3) pseudokinase plays a crucial role in cell proliferation, migration and morphogenesis during development. In our recent study, an introduction of human TRB3 gene into mouse mammary tumor cells caused an increase of proliferation of tumor cells and their nuclear size. In the current study, to examine whether this gene causes <i>de novo</i> morphological changes in a specific organ site we have developed a novel variation of the transgenic mouse model that conditionally expresses human TRB3 (hTRB3) gene using Cre-recombinase (Cre)/loxP recombination system. By injecting hTRB3 transgene construct into pronuclei of mouse embryo, we eventually obtained four hTRB3 mice. The gene expression was controlled by infection of adenovirus-expressing Cre <i>via</i> the tail vein of hTRB3 mouse. In Cre-mediated hTRB3 mouse, expression of the hTRB3 protein was detected in the cytoplasm of hepatocytes in the liver. Expression of this protein was also seen in lymphocytes in the spleen, glomerular endothelial cells, and epithelial cells of collecting duct of the kidney. In hepatocytes of the hTRB3 mouse, nuclear size was significantly greater than that of the wild type mouse, indicating that hTRB3 can play a role at least in part in hepatic morphogenesis. The present animal model may provide a system for evaluation of <i>de novo</i> morphological changes induced by a specific transgene in a specific organ site.

Journal

  • Biological and Pharmaceutical Bulletin

    Biological and Pharmaceutical Bulletin 37(6), 1068-1074, 2014

    The Pharmaceutical Society of Japan

Codes

  • NII Article ID (NAID)
    130004147355
  • NII NACSIS-CAT ID (NCID)
    AA10885497
  • Text Lang
    ENG
  • ISSN
    0918-6158
  • NDL Article ID
    025479017
  • NDL Call No.
    Z53-V41
  • Data Source
    NDL  J-STAGE 
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