Total Flavonoids of Litsea Coreana Enhance the Cytotoxicity of Oxaliplatin by Increasing Gap Junction Intercellular Communication
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- Yu Bin-bin
- Department of Pharmacy, Bengbu Medical College
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- Dong Shu-ying
- Department of Pharmacy, Bengbu Medical College
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- Yu Mei-ling
- Department of Pharmacy, The First Affiliated Hospital of Bengbu Medical College
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- Jiang Guo-jun
- Department of Pharmacy, Bengbu Medical College
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- Ji Jie
- Department of Pharmacy, Bengbu Medical College
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- Tong Xu-hui
- Department of Pharmacy, Bengbu Medical College
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抄録
Oxaliplatin is widely used in the treatment of variety of cancers, including cancer of the testis and colorectum. Gap junctions (GJs) can amplify the cytotoxicity of antinoeoplastic drugs through the bystander effect in different cancer cells. In this study, we demonstrate that total flavonoids of litsea coreana (TFLC), one extract from the dried leaves of litsea coreana leve, increase the cytotoxicity of oxaliplatin in mouse testicular cancer I-10 cells. We found that cell survival was substantially decreased only when functional GJs formed in I-10 cells. TFLC increased oxaliplatin cytotoxity (inducing cell death and apoptosis) by enhancing gap junction intercellular communication (GJIC) through elevated Cx43 protein expression. Furthermore, apoptosis-related protein (Bax, Bcl-2, caspase-3/9) results showed that the Bax/Bcl-2 ratio and activated caspase-3/9 increased when TFLC was used compared with treatment with oxaliplatin alone, which suggests that the mechanism of increased oxaliplatin-induced apoptosis was through the mitochondrial pathway. These results demonstrate that TFLC can enhance the cytotoxicity of oxaliplatin, and that these processes may be regulated in testicular tumor cells through GJ-mediated regulation of tumor cell apoptosis.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 37 (8), 1315-1322, 2014
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204634363136
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- NII論文ID
- 130004147358
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC2cjmslOhtA%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 025609963
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- PubMed
- 24871203
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可