Artificial Reproduction of Kuchinoshima Wild Cattle

DOI

Bibliographic Information

Other Title
  • 口之島野生化牛の人口繁殖

Abstract

In this study, we attempted the application of several biotechnology techniques to the Kuchinoshima wild cattle, endangered Japanese cattle.<BR>On a result of measurement of their shapes, it was rather small in stature than that of Japanese black. Five Kuchinoshima wild cattle, four Japanese black and two F1 (Holstein × Japanese black) cattle were inseminated artificially by using frozen-thawed epididymal spermatozoa of wild cattle which had been cryopreserved by standard technique in a liquid nitrogen (LN2) . Seven out of 11 cows (including 3 wild cattle) became pregnant. When in vitro matured oocytes collected from Japanese black were fertilized with frozen-thawed epididymal spermatozoa of wild cattle, 69.3% (296/427) oocytes cleaved and 25.8% (110/427) of them developed to blastocysts. Embryo collections were conducted on three superovulated wild cattle which were inseminated with frozen-thawed ejaculated spermatozoa of Japanese black, but only one of them produced transferable embryos. Embryo transfers were conducted on 3 recipient cows and one of them became pregnant.

Journal

Details 詳細情報について

  • CRID
    1390282679419842048
  • NII Article ID
    130004185199
  • DOI
    10.11461/jwaras1968.39.43
  • ISSN
    18846394
    09143459
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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