マウスにおける子宮内膜内微小環境修飾による着床率の改善

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  • Modification of the local milieu surrounding the embryo improves the implantation rate in mice.

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The aim of this study is to clarify whether modification of the local milieu surrounding the embryo improves the implantation rate. It has been demonstrated that pregnancy can be achieved by the direct insertion of embryos into the endometrium of mice. To modify the local milieu surrounding the embryo, we inserted blastocysts together with various bioactive substances into the endometrium of pseudopregnant mice. The implantation rates were evaluated 8 days after embryo transfer. The substances tested were histamine, prostaglandinE2, progesterone and human chorionic gonadotropin. Among them, only histamine exerted the effect on the embryo implantation. The implantation rate was improved on day 2 (31.5 %) when embryos were transferred together with histamine (100pM). A study to clarify the mechanism of this improvement by histamine is now under way. So far, histamine has had no effect on blastocyst development in vitro in terms of trophoectoderm outgrowth or on decidualization in an endometrial stromal cell culture. Marked stromal edema was found around the embryos transferred with histamine. Histamine may enhance implantation not by directly affecting embryos and decidualization in the stroma but by modifying the local milieu surrounding embryos as a result of increased vascular permeability. [Adv Obstet Gynecol 49 (5); 516---527, 1997 (H9.9)]

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