プラセンターエキスに含有される酵素アルカリホスファターゼのマイクロカプセル化とその特性

• 近松 義博

  一丸貿易株式会社製造開発室

• 安藤 裕

  一丸貿易株式会社製造開発室

• 川島 嘉明

  岐阜薬科大学製剤学研究室

書誌事項

タイトル別名

• Characteristic of the microencapsulated alkaline phosphatase

抄録

Alkaline phosphatase was microcapsulated to be immobilized with stylene polymer by employing a drying in liquid method. The size distribution of the microcapsules was a bimodal form. The larger microcapsules had the stronger activities and were the more dense than the one with the smaller size. The optimum temperature and pH for the reaction of the microencapsulated enzyme shifted to the higher ranges than the native enzyme. The reaction rate of the microcapsules because delayed significantly. The reaction process was analyzed by Lineweaver-Burk plots of data to test the Michael-Menten equation. It was found that the reaction rate was controlled by not only the diffusion of donor in the microcapsule but also a cappillary flow of the enzyme solution through the pin holes on the surface of the microcapsule, which were observed by a scanning electron microscope. The latter was proved by the fact that the reaction rate was affected by agitation speed and the reaction proceeded still more even if the microcasules were removed from the system.

収録刊行物

• 日本化粧品技術者会誌

  日本化粧品技術者会誌 14 (2), 138-145, 1980

  日本化粧品技術者会

詳細情報

• CRID: 1390001204499877376
• NII論文ID: 130004188491
• DOI: 10.5107/sccj.14.138
• ISSN: 18844146
• ISSN: 03875253
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可