高速液体クロマトグラフ法によるCefbuperazoneのかくたん中濃度測定の検討  [in Japanese] Quantitative analysis of cefbuperazone in sputum by high performance liquid chromatography (HPLC).  [in Japanese]

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Abstract

新cephamycln系抗生剤Cefbuperazone (CBPZ) の喀痰中濃度測定法について, 高速液体クロマトグラフィー (HPLC法) の適用と改良を行ない, ヒトでのCBPZの喀痰中移行を検討した。<BR>(1) HPLC法について, 喀痰の前処理として粘稠性物質および測定妨害物質の除去を行ない, さらに測定波長を選択することにより, 喀痰中CBPZ濃度の測定が可能となり, 生物学的測定法と良く一致した測定値が得られ, その相関係数は0.940であった。<BR>(2) 5名の呼吸器疾患患者にCBPZ 1gおよび29を1時間点滴静注投与後の喀痰中CBPZ濃度が測定された。各患者ごとのピーク値および経時的パターンとも個人差が著しかったが, 投与量により各個人ごとに明らかなDose responseがみられ, そのピーク値は1g投与で1.2~2.4μg/ml, 29投与で1.9~5.0μg/mlの喀痰中濃度が得られた。

The concentration of cefbuperazone (CBPZ), a new cephamycin antibiotic, in human sputum after administration has been quantitatively analysed using bioassay and HPLC techniques which was applied and improved in this study.<BR>(1) By the removals of mucus and the obstructive substance from sputum as a pretreatment procedure, and the improvement of measurement condition through the selection of monitored wavelength, the quantitative analysis by HPLC was well controlled and coincided with those by bioassay, resulted in the coefficient of correlation being 0.940.<BR>(2) The concentration of CBPZ in sputum was analysed in five patients with pulmonary disease who were given with 1g and 2g via a drip-intravenous infusion for 1 hr. The maximum peak and pattern of concentrations with time of the individual. maximum concentration in sputum was detected in the range of 1.2-2.4μg/ml for 1g dose and 1.9-5.0μg/ml for 2g dose at a dose dependent manner.

Journal

  • CHEMOTHERAPY

    CHEMOTHERAPY 33(1), 24-31, 1985

    Japanese Society of Chemotherapy

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