TE-031の体液内濃度測定法に関する研究 (第1報)

DOI

書誌事項

タイトル別名
  • ASSAYS FOR TE-031 (A-56268)
  • IN BODY FLUIDS (I) MICROBIOLOGICAL ASSAY
  • Bioassay法による体液内濃度測定

抄録

To establish a bioassay for a new antibiotic, TE-031 (A-56268), in body fluids, various methods and conditions were studied. The most suitable assay used a disc plate method with Micrococcus luteus ATCC 9341 as test organism in heart infusion agar, pH 8.0. As standard solution of TE-031, consera for human serum, methanol phosphate buffer (methanol: 0.02M phosphate buffer, pH 7.4=4:1) and partly 1/15M phosphate buffer, pH 7.2 for urine, and methanol phosphate buffer for tissue were used. Samples were prepared by diluting the above standard solutions.<BR>The most active metabolite of TE-031, M-5, was also measured by the same bioassay. Its standard curve was almost the same as that of TE-031. The detectable range of TE-031 concentrations was 0.025-100μg/ml (except 1/15M phosphate buffer: 0.2-100μg/ml). There was good correlation between the bioassay and chemical assays.

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