TE-031の体液内濃度測定法に関する研究 (第1報)
書誌事項
- タイトル別名
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- ASSAYS FOR TE-031 (A-56268)
- IN BODY FLUIDS (I) MICROBIOLOGICAL ASSAY
- Bioassay法による体液内濃度測定
抄録
To establish a bioassay for a new antibiotic, TE-031 (A-56268), in body fluids, various methods and conditions were studied. The most suitable assay used a disc plate method with Micrococcus luteus ATCC 9341 as test organism in heart infusion agar, pH 8.0. As standard solution of TE-031, consera for human serum, methanol phosphate buffer (methanol: 0.02M phosphate buffer, pH 7.4=4:1) and partly 1/15M phosphate buffer, pH 7.2 for urine, and methanol phosphate buffer for tissue were used. Samples were prepared by diluting the above standard solutions.<BR>The most active metabolite of TE-031, M-5, was also measured by the same bioassay. Its standard curve was almost the same as that of TE-031. The detectable range of TE-031 concentrations was 0.025-100μg/ml (except 1/15M phosphate buffer: 0.2-100μg/ml). There was good correlation between the bioassay and chemical assays.
収録刊行物
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- CHEMOTHERAPY
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CHEMOTHERAPY 36 (Supplement3), 170-191, 1988
公益社団法人 日本化学療法学会
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詳細情報 詳細情報について
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- CRID
- 1390001206285941248
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- NII論文ID
- 130004196185
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- ISSN
- 18845894
- 00093165
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可