酢酸菌とその利用に関する研究 (第3報)

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タイトル別名
  • Studies on acetic acid bacteria and their utilization (Part III)
  • ピロ タンサン ジエチルエステル ニ ヨル ショクズ ノ コンダク ボウシ ニ ツイテ
  • Prevention of Clouding of Vinegar by DEPC
  • ピロ炭酸ジエチルエステルによる食酢の溷濁防止について

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Experiments were carried out chiefly on comparison of anticeptic efficacy of DEPC and efficacy of anticeptics whose use are permitted at present.<BR>Acetic acid bacteria is an aerobic bacteria and as it is difficult to count the cells because they form cluster, 1% of surface active agent was added to disperse the cells of the bacteria, by which the cells disperse easily and cell counting becomes easy.<BR>Next, the kinds of acetic acid bacteria and the sterilizing effect were investigated by using yeast and mold isolated from vinegar but no difference was observed with the exception of mold. Also, it was possible to sterilize acetic acid bacteria present in vinegar in the order of 103 with 25 ppm of DEPC but the sterilizing effect became lower as the acid concentration of the vinegar became lower and it was possible to sterilize completely with DEPC concentration of 50 ppm in case of the ordinary vinegar acid concentration and when treated for 24 hours with a bacteria count in the order of 103-106. Actually, it was found that DEPC concentration of 100 ppm was repuired for complete sterilization of clouding bacteria present in the order of 104-106 in the vinegar.<BR>Anticeptic agents SA and POBB whose use are permitted at present were almost completely ineffective even when the maximum permissible abounts were used. However, it was found that POBB is more effective as a bacteriostatic agent than as a sterilizing agent.<BR>DEPC has a temporary sterilizing effect but as it does not maintain this effect very long, other food preservatives were added and although there were no problems when kept sealed, the vinegar was reinnoculated after sterilizing with DEPC on the assumption that recontamination takes place and as a result, it was found that propagation of bacteria took place and complete preservation was not possible.

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