Azurin–DNA Conjugate with the Binding Motif of a Transcriptional Regulator, CooA: CO-dependent Modulation of the Electron-transfer Reaction
Access this Article
An azurin–DNA conjugate (Azu–DNA) where the DNA part contains the binding sequence of a carbon monoxide (CO)-dependent transcriptional regulator, CooA, has been prepared. CooA in the CO-bound form (CO–CooA) showed specific binding to Azu–DNA. The association of CO–CooA with Azu–DNA affects the electron transfer (ET) between Azu–DNA and its redox partner protein, cytochrome <i>c</i><sub>552</sub>. Consequently, the CO-dependent modulation of ET was achieved. This could be a potential platform for a novel biobased sensor, which exploits the transcriptional regulator as a detection module and whose readout is immediately transduced to a readily processable electronic signal.
- Chemistry Letters
Chemistry Letters 43(8), 1204-1206, 2014
The Chemical Society of Japan