Azurin–DNA Conjugate with the Binding Motif of a Transcriptional Regulator, CooA: CO-dependent Modulation of the Electron-transfer Reaction

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Author(s)

Abstract

An azurin–DNA conjugate (Azu–DNA) where the DNA part contains the binding sequence of a carbon monoxide (CO)-dependent transcriptional regulator, CooA, has been prepared. CooA in the CO-bound form (CO–CooA) showed specific binding to Azu–DNA. The association of CO–CooA with Azu–DNA affects the electron transfer (ET) between Azu–DNA and its redox partner protein, cytochrome <i>c</i><sub>552</sub>. Consequently, the CO-dependent modulation of ET was achieved. This could be a potential platform for a novel biobased sensor, which exploits the transcriptional regulator as a detection module and whose readout is immediately transduced to a readily processable electronic signal.

Journal

  • Chemistry Letters

    Chemistry Letters 43(8), 1204-1206, 2014

    The Chemical Society of Japan

Codes

  • NII Article ID (NAID)
    130004427118
  • Text Lang
    ENG
  • ISSN
    0366-7022
  • Data Source
    J-STAGE 
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