Azurin–DNA Conjugate with the Binding Motif of a Transcriptional Regulator, CooA: CO-dependent Modulation of the Electron-transfer Reaction
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- Hiroshi Nakajima
- Graduate School of Science, Nagoya University
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- Soji Miyazaki
- Research Centre of Materials Science, Nagoya University
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- Takaaki Itoh
- Graduate School of Science, Nagoya University
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- Mao Hayamura
- Graduate School of Science, Nagoya University
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- Yoshihito Watanabe
- Research Centre of Materials Science, Nagoya University
抄録
<jats:title>Abstract</jats:title> <jats:p>An azurin–DNA conjugate (Azu–DNA) where the DNA part contains the binding sequence of a carbon monoxide (CO)-dependent transcriptional regulator, CooA, has been prepared. CooA in the CO-bound form (CO–CooA) showed specific binding to Azu–DNA. The association of CO–CooA with Azu–DNA affects the electron transfer (ET) between Azu–DNA and its redox partner protein, cytochrome c552. Consequently, the CO-dependent modulation of ET was achieved. This could be a potential platform for a novel biobased sensor, which exploits the transcriptional regulator as a detection module and whose readout is immediately transduced to a readily processable electronic signal.</jats:p>
収録刊行物
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- Chemistry Letters
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Chemistry Letters 43 (8), 1204-1206, 2014-05-01
Oxford University Press (OUP)
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詳細情報 詳細情報について
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- CRID
- 1360846644035667072
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- NII論文ID
- 130004427118
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- ISSN
- 13480715
- 03667022
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