Prevalence of Virulent Rhodococcus equi in Soil Environment on a Horse-Breeding Farm in Tennessee, U.S.A.

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Author(s)

    • TAKAI Shinji
    • Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • YAMATODA Noriko
    • Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • KIMURA Ayumi
    • Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • SASAKI Yukako
    • Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • KAKUDA Tsutomu
    • Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • TSUBAKI Shiro
    • Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University
    • BEMIS David
    • College of Veterinary Medicine, University of Tennessee

Abstract

Virulent Rhodococcus equi is an important pathogen in foals aged 1 to 3 months, but its incidence in Tennessee, U. S. A., is poorly understood. Ten soil samples were collected from a Tennessee walking horse farm in Tennessee. The presence of R. equi was investigated and 107 isolates were tested for the presence of 15- to 17-kDa antigens (VapA) by immunoblotting and PCR. R. equi was isolated (10<sup>3</sup>-10<sup>4</sup> colony forming units/g) from 8 of the 10 soil samples. Twenty-four VapA-positive isolates were detected from 7 of the 8 culture-positive samples with a prevalences ranging from 6.7 to 50%. Of the 24 virulent isolates, 21 contained an 85-kb type I plasmid, and 3 contained an 85-kb type III plasmid which had been uniquely isolated from soil isolates in Texas. There was no evidence of R. equi infection on the farm, but the virulent form was widespread in the soil environment of the farm.<br>

Journal

  • Journal of Equine Science

    Journal of Equine Science 15(3), 75-79, 2004

    Japanese Society of Equine Science

Codes

  • NII Article ID (NAID)
    130004431209
  • Text Lang
    ENG
  • ISSN
    1340-3516
  • Data Source
    J-STAGE 
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