Prevalence of Virulent Rhodococcus equi in Soil Environment on a Horse-Breeding Farm in Tennessee, U.S.A.

• TAKAI Shinji

  Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University

• YAMATODA Noriko

  Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University

• KIMURA Ayumi

  Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University

• SASAKI Yukako

  Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University

• KAKUDA Tsutomu

  Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University

• TSUBAKI Shiro

  Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University

• BEMIS David

  College of Veterinary Medicine, University of Tennessee

• BRACE James J.

  College of Veterinary Medicine, University of Tennessee

Abstract

Virulent Rhodococcus equi is an important pathogen in foals aged 1 to 3 months, but its incidence in Tennessee, U. S. A., is poorly understood. Ten soil samples were collected from a Tennessee walking horse farm in Tennessee. The presence of R. equi was investigated and 107 isolates were tested for the presence of 15- to 17-kDa antigens (VapA) by immunoblotting and PCR. R. equi was isolated (10³-10⁴ colony forming units/g) from 8 of the 10 soil samples. Twenty-four VapA-positive isolates were detected from 7 of the 8 culture-positive samples with a prevalences ranging from 6.7 to 50%. Of the 24 virulent isolates, 21 contained an 85-kb type I plasmid, and 3 contained an 85-kb type III plasmid which had been uniquely isolated from soil isolates in Texas. There was no evidence of R. equi infection on the farm, but the virulent form was widespread in the soil environment of the farm.<br>

Journal

• Journal of Equine Science

  Journal of Equine Science 15 (3), 75-79, 2004

  Japanese Society of Equine Science

Keywords

• foal
• Rhodococcus equi
• soil
• Tennessee

Details

• CRID: 1390001204369484928
• NII Article ID: 30002175463
• NII Article ID: 130004431209
• NII Article ID: 10014018545
• NII Book ID: AA11010806
• DOI: 10.1294/jes.15.75
• ISSN: 13477501
• ISSN: 13403516
• NDL BIB ID: 7151260
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7151260
• Web Site: http://www.jstage.jst.go.jp/article/jes/15/3/15_3_75/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2005045716
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed