Augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats
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- Sakai Hiroyasu
- Department of Pharmacology, Hoshi University, Tokyo, Japan Division of Pharmacy Professional Development and Research, Hoshi University, Tokyo, Japan
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- Watanabe Ayako
- Department of Pharmacology, Hoshi University, Tokyo, Japan
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- Fujita Akiko
- Department of Pharmacology, Hoshi University, Tokyo, Japan
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- Misawa Miwa
- Department of Pharmacology, Hoshi University, Tokyo, Japan
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- Narita Minoru
- Department of Pharmacology, Hoshi University, Tokyo, Japan
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- Chiba Yoshihiko
- Department of Biology, School of Pharmacy, Hoshi University, Tokyo, Japan
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Abstract
Cigarette smoking is the main risk factor for the development of chronic obstructive pulmonary disease (COPD). However, little is known about the mechanisms of cigarette smoke-induced bronchial smooth muscle (BSM) hyperresponsiveness. In the present study, we investigated the effects of aqueous cigarette smoke extract (ACSE) on the BSM contraction in rats. The bronchial strips of rats were incubated with ACSE or control-extract for 24 h. The acetylcholine (ACh), high K+ depolarization and sodium fluoride (NaF)-induced BSM contraction of the ACSE-treated group was significantly augmented as compared to that of the control one. The expression levels of both myosin light-chain kinase (MLCK) and RhoA were significantly increased in the ACSE-treated BSM. These findings suggest that the water-soluble components of cigarette smoke may cause BSM hyperresponsiveness via an increase in MLCK and RhoA.<br>
Journal
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- Journal of Smooth Muscle Research
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Journal of Smooth Muscle Research 50 (0), 39-47, 2014
Japan Society of Smooth Muscle Research